Characterization of Enterococcus faecalis isolates originating from different sources for their virulence factors and genes, antibiotic resistance patterns, genotypes and biofilm production

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dc.authorid0000-0001-8370-8677
dc.authorid0000-0002-2967-213X
dc.authorscopusid6507512220
dc.authorscopusid6603188754
dc.authorscopusid34980023500
dc.authorscopusid36025889700
dc.authorscopusid32867692200
dc.authorwosidCIFTCI, ALPER/AAD-9374-2022
dc.authorwosidBOYNUKARA, banur/ABA-3405-2020
dc.contributor.authorGülhan, Timur
dc.contributor.authorBoynukara, Banur
dc.contributor.authorÇiftçi, Alper
dc.contributor.authorSöğüt, Mehtap Ünlü
dc.contributor.authorFındık, Arzu
dc.date.accessioned2022-05-11T14:42:19Z
dc.date.available2022-05-11T14:42:19Z
dc.date.issued2015
dc.departmentFakülteler, Veteriner Fakültesi, Klinik Öncesi Bilimler Bölümü, Mikrobiyoloji Ana Bilim Dalı
dc.description.abstractIn this study, 72 Enterococcus faecalis isolates originating from humans (n=39), dogs (n=26) and cats (n=7) were investigated for some virulence factors, some virulence genes, antibiotic resistance phenotypes, randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) patterns and biofilm production. Of the isolates, 31 (43.1%) were positive for gelatinase, 11(15.3%) for aggregation substance and cytolysine, 38 (52.8%) for gelE and 34 (47.2%) for asal genes. All isolates were found to be negative for hyl, esp and cylA genes. All isolates were found to be resistant to nalidixic acid and kanamycin. On the other hand, all isolates were cited for susceptible to amoxicillin. Vancomycin resistance genes (vanA, vanB, vanC I/C2 or vanD) have not been detected in any of the phenotypically vancomycin resistant isolates. Isolates from humans, dogs and cats were grouped into 8, 2 and 4 antibiotypes depending upon susceptibilities to 12 different antibiotics. In all human, dog and cat isolates, 9, 12 and 2 genotypes were determined by RAPD-PCR, respectively. Nine (34.6%) of the dog isolates were found to be positive for biofilm production. This study showed that multiple antibiotic resistance among human isolates is more frequent than in dog and cat isolates.
dc.identifier.endpage266
dc.identifier.issn1728-1997
dc.identifier.issue3en_US
dc.identifier.pmid27175186
dc.identifier.scopus2-s2.0-84945308893
dc.identifier.scopusqualityQ2
dc.identifier.startpage261
dc.identifier.urihttps://hdl.handle.net/20.500.11776/9330
dc.identifier.volume16
dc.identifier.wosWOS:000367865800004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorBoynukara, Banur
dc.language.isoen
dc.publisherShiraz Univ
dc.relation.ispartofIranian Journal of Veterinary Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAntibiotyping
dc.subjectBiofilm
dc.subjectEnterococcus faecalis
dc.subjectGenotyping
dc.subjectVirulence
dc.subjectEsp Gene
dc.subjectVancomycin
dc.subjectStrains
dc.subjectFaecium
dc.subjectHumans
dc.subjectSusceptibility
dc.subjectDeterminants
dc.subjectAnimals
dc.subjectDogs
dc.subjectPcr
dc.titleCharacterization of Enterococcus faecalis isolates originating from different sources for their virulence factors and genes, antibiotic resistance patterns, genotypes and biofilm production
dc.typeArticle

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