A novel rhabdovirus, related to Merida virus, in field-collected mosquitoes from Anatolia and Thrace

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dc.authorid0000-0001-5422-1982
dc.authorid0000-0002-2583-6264
dc.authorid0000-0002-9711-4113
dc.authorid0000-0001-8185-3176
dc.authorscopusid6507415764
dc.authorscopusid55258915300
dc.authorscopusid23566817100
dc.authorscopusid37114339500
dc.authorscopusid15769247000
dc.authorscopusid36968366100
dc.authorscopusid55881292900
dc.authorwosidLinton, Yvonne/AAG-2353-2019
dc.authorwosidLinton, Yvonne-Marie/AAW-3992-2021
dc.authorwosidGunay, Filiz/AAC-7874-2020
dc.authorwosidErgunay, Koray/I-8368-2013
dc.contributor.authorErgünay, Koray
dc.contributor.authorBrinkmann, Annika
dc.contributor.authorLitzba, Nadine
dc.contributor.authorGunay, Filiz
dc.contributor.authorKar, Sırrı
dc.contributor.authorÖter, Kerem
dc.contributor.authorLinton, Yvonne-Marie
dc.date.accessioned2022-05-11T14:28:36Z
dc.date.available2022-05-11T14:28:36Z
dc.date.issued2017
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractNext-generation sequencing technologies have significantly facilitated the discovery of novel viruses, and metagenomic surveillance of arthropods has enabled exploration of the diversity of novel or known viral agents. We have identified a novel rhabdovirus that is genetically related to the recently described Merida virus via next-generation sequencing in a mosquito pool from Thrace. The complete viral genome contains 11,798 nucleotides with 83% genome-wide nucleotide sequence similarity to Merida virus. Five major putative open reading frames that follow the canonical rhabdovirus genome organization were identified. A total of 1380 mosquitoes comprising 13 species, collected from Thrace and the Mediterranean and Aegean regions of Anatolia were screened for the novel virus using primers based on the N and L genes of the prototype genome. Eight positive pools (6.2%) exclusively comprised Culex pipiens sensu lato specimens originating from all study regions. Infections were observed in pools with female as well as male or mixed-sex individuals. The overall and Cx. pipiens-specific minimal infection rates were calculated to be 5.7 and 14.8, respectively. Sequencing of the PCR products revealed marked diversity within a portion of the N gene, with up to 4% divergence and distinct amino acid substitutions that were unrelated to the collection site. Phylogenetic analysis of the complete and partial viral polymerase (L gene) amino acid sequences placed the novel virus and Merida virus in a distinct group, indicating that these strains are closely related. The strain is tentatively named Merida-like virus Turkey. Studies are underway to isolate and further explore the host range and distribution of this new strain.
dc.description.sponsorshipArmed Forces Health Surveillance Center, Global Emerging Infections Surveillance and Response System (AFHSC-GEIS), United States [W911QY-16-C-0160-P00001]
dc.description.sponsorshipThis study was partially supported by the Armed Forces Health Surveillance Center, Global Emerging Infections Surveillance and Response System (AFHSC-GEIS), United States (with Yvonne Marie-Linton as the principal investigator) (grant agreement number: W911QY-16-C-0160-P00001).
dc.identifier.doi10.1007/s00705-017-3314-4
dc.identifier.endpage1911
dc.identifier.issn0304-8608
dc.identifier.issn1432-8798
dc.identifier.issue7en_US
dc.identifier.pmid28283817
dc.identifier.scopus2-s2.0-85014777633
dc.identifier.scopusqualityQ2
dc.identifier.startpage1903
dc.identifier.urihttps://doi.org/10.1007/s00705-017-3314-4
dc.identifier.urihttps://hdl.handle.net/20.500.11776/6882
dc.identifier.volume162
dc.identifier.wosWOS:000403454900007
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKar, Sırrı
dc.language.isoen
dc.publisherSpringer Wien
dc.relation.ispartofArchives of Virology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectWest Nile Virus
dc.subjectAlignment
dc.subjectSurveillance
dc.subjectCirculation
dc.titleA novel rhabdovirus, related to Merida virus, in field-collected mosquitoes from Anatolia and Thrace
dc.typeArticle

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