Short-term humoral immune response of the pcDNA4-G plasmid expressing the bovine ephemeral fever virus G gene in BALB/c mice

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dc.authorid0000-0003-2116-105X
dc.authorscopusid57103691500
dc.authorscopusid7003605484
dc.authorwosidAbayli, Hasan/W-1858-2018
dc.contributor.authorAbaylı, Hasan
dc.contributor.authorBulut, Hakan
dc.date.accessioned2022-05-11T14:42:22Z
dc.date.available2022-05-11T14:42:22Z
dc.date.issued2021
dc.departmentFakülteler, Veteriner Fakültesi, Klinik Öncesi Bilimler Bölümü, Viroloji Ana Bilim Dalı
dc.description.abstractBovine ephemeral fever (BEF) is an arthropod-borne viral disease characterised by a short-term clinical expression that can lead to significant losses in high-yielding cattle and water buffaloes. In this study, we aimed to generate a recombinant plasmid expressing the glycoprotein (G) of the BEF virus (BEFV) and to stimulate a humoral immune response to this protein in BALB / c mice immunised with the recombinant plasmid. Expression of the encoded protein was demonstrated by western blotting and immunoperoxidase tests. The suitable plasmids were intramuscularly administered to BALB/c mice on days 0, 14 and 21. The antibody response in the immunised mice was measured by a plaque reduction neutralization test (PRNT) and enzyme-linked immunosorbent assay (ELISA). According to BEFV ELISA, only two of the seven animals in these groups exceeded the cut-off value. A significant difference was observed in the mean OD values at 450 nm absorbance in the pcDNA4-G-immunised group when compared with those in the plasmid control group at 30 days (p < 0.05). According to PRNT50 results, a 1:20 (p < 0.05) antibody response was obtained at 30 days in pcDNA4-G (100 mu g)-immunised mice, whereas this ratio was 1:80 (p < 0.001) in BEFV-immunised mice (1,000 PFU/0.5 ml). We conclude that the humoral immune response was stimulated in experimental mice immunised with the recombinant plasmid. However, disappointingly, the antibody response was markedly low in pcDNA4-G-immunised mice.
dc.description.sponsorshipScientific Research Projects Coordination Unit of Firat UniversityFirat University [VF1627]
dc.description.sponsorshipThis work was supported by project number VF1627 by the Scientific Research Projects Coordination Unit of Firat University.
dc.identifier.doi10.12834/VetIt.2077.12075.1
dc.identifier.endpage150
dc.identifier.issn0505-401X
dc.identifier.issn1828-1427
dc.identifier.issue2en_US
dc.identifier.pmid34971503
dc.identifier.scopus2-s2.0-85123037285
dc.identifier.scopusqualityQ2
dc.identifier.startpage143
dc.identifier.urihttps://doi.org/10.12834/VetIt.2077.12075.1
dc.identifier.urihttps://hdl.handle.net/20.500.11776/9351
dc.identifier.volume57
dc.identifier.wosWOS:000754628600007
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorBulut, Hakan
dc.language.isoen
dc.publisherIst Zooprofilattico Sperimentale Abruzzo & Molise G Caporale-Izs A&M
dc.relation.ispartofVeterinaria Italiana
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAntibody response
dc.subjectBovine ephemeral fever
dc.subjectDNA vaccine
dc.subjectDna Vaccines
dc.subjectNeutralizing Antibodies
dc.subjectDisease Virus
dc.subjectGlycoprotein
dc.subjectProtects
dc.titleShort-term humoral immune response of the pcDNA4-G plasmid expressing the bovine ephemeral fever virus G gene in BALB/c mice
dc.typeArticle

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