Isolation and genomic characterization of Culex theileri flaviviruses in field-collected mosquitoes from Turkey

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dc.authorid0000-0002-2583-6264
dc.authorid0000-0001-5422-1982
dc.authorid0000-0001-8185-3176
dc.authorid0000-0002-9711-4113
dc.authorscopusid6507415764
dc.authorscopusid23566817100
dc.authorscopusid55258915300
dc.authorscopusid37114339500
dc.authorscopusid15769247000
dc.authorscopusid36968366100
dc.authorscopusid55881292900
dc.authorwosidGunay, Filiz/AAC-7874-2020
dc.authorwosidLinton, Yvonne/AAG-2353-2019
dc.authorwosidErgunay, Koray/I-8368-2013
dc.authorwosidLinton, Yvonne-Marie/AAW-3992-2021
dc.contributor.authorErgünay, Koray
dc.contributor.authorLitzba, Nadine
dc.contributor.authorBrinkmann, Annika
dc.contributor.authorGunay, Filiz
dc.contributor.authorKar, Sırrı
dc.contributor.authorÖter, Kerem
dc.contributor.authorLinton, Yvonne-Marie
dc.date.accessioned2022-05-11T14:28:34Z
dc.date.available2022-05-11T14:28:34Z
dc.date.issued2016
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractVector surveillance for the arthropod-borne infections has resulted in the isolation of a growing number of novel viruses, including several flavivirus strains that exclusively replicate in insects. This report describes the isolation and genomic characterization of four insect-specific flaviviruses frommosquitoes, previously collected from various locations in Turkey. C6/36 Aedes albopictus and Vero cell lines were inoculated with mosquito pools. On C6/36 cells, mild cytopathic effects, characterized as rounding and detachment, were observed in four pools that comprised female Culex theileri mosquitoes. Complete (3 isolates, 10,697 nucleotides) or near-complete (1 isolate, 10,452 nucleotides) genomic characterization was performed in these culture supernatants via next generation sequencing. All strains demonstrated high genetic similarities, with over 99% identity match on nucleotide and amino acid alignments, revealing them to be different isolates of the same virus. Sequence comparisons identified the closest relative to be the Culex theileri flavivirus (CTFV) strains, originally characterized in Portugal. Phylogenetic analyses demonstrated that the isolates remained distinct as a cluster but formed amonophyletic group with CTFV strains, and shared a common ancestor with Quang Binh or related Culex flaviviruses. The organization of the viral genome was consistent with the universal flavivirus structure and stem-loops; conserved motifs and imperfect tandem repeats were identified in the non-coding ends of the viral genomes. A potential ribosomal shifting site, resulting in the translation of an additional reading frame, was detected. The deduced viral polyprotein comprised 3357 amino acids and was highly-conserved. Amino acid variations, presumably associated with adaptive environmental pressures, were identified. These isolates comprise the first fully characterized insect-specific flaviviruses in Turkey. Their impact on West Nile virus circulation, which is also endemic in the study region, remains to be explored. (C) 2016 Elsevier B.V. All rights reserved.
dc.description.sponsorshipArmed Forces Health Surveillance Center, Global Emerging Infections Surveillance and Response System (AFHSC-GEIS), United States [W81XWH-11-2-0174]; Georg Forster Research Fellowship (HERMES) for Experienced Researchers by Alexander von Humboldt Foundation; National Research Council (NRC) Research Associateship Award at the Walter Reed Army Institute of Research
dc.description.sponsorshipThis study was partially supported by The Armed Forces Health Surveillance Center, Global Emerging Infections Surveillance and Response System (AFHSC-GEIS), United States (W81XWH-11-2-0174) (with Yvonne-Marie Linton as the principal investigator). KE is a recipient of the Georg Forster Research Fellowship (HERMES) for Experienced Researchers by the Alexander von Humboldt Foundation, 2015. This manuscript was prepared whilst YML held a National Research Council (NRC) Research Associateship Award at the Walter Reed Army Institute of Research. This research was performed in part under a Memorandum of Understanding between the Walter Reed Army Institute of Research and the Smithsonian Institution, with institutional support provided by both organizations. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. The material to be published reflects the views of the authors and should not be construed to represent those of the US Department of the Army or the US Department of Defense.
dc.identifier.doi10.1016/j.meegid.2016.11.008
dc.identifier.endpage147
dc.identifier.issn1567-1348
dc.identifier.issn1567-7257
dc.identifier.pmid27840255
dc.identifier.scopus2-s2.0-84995576489
dc.identifier.scopusqualityQ1
dc.identifier.startpage138
dc.identifier.urihttps://doi.org/10.1016/j.meegid.2016.11.008
dc.identifier.urihttps://hdl.handle.net/20.500.11776/6873
dc.identifier.volume46
dc.identifier.wosWOS:000390456000021
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKar, Sırrı
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofInfection Genetics and Evolution
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectFlavivirus
dc.subjectMosquito
dc.subjectCulex
dc.subjectTurkey
dc.subjectGenome
dc.subjectGenetic-Characterization
dc.subjectAlignment
dc.subjectRna
dc.subjectSurveillance
dc.subjectEvolution
dc.subjectViruses
dc.titleIsolation and genomic characterization of Culex theileri flaviviruses in field-collected mosquitoes from Turkey
dc.typeArticle

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