Evaluation of Antioxidant activities of Brassica napus's seeds by CUPRAC, ABTS/Persulphate and DMPD methods

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dc.authorid0000-0003-2759-4411
dc.authorwosidkarasakal, ayca/ABA-4762-2020
dc.contributor.authorKarasakal, Ayça
dc.date.accessioned2022-05-11T14:30:52Z
dc.date.available2022-05-11T14:30:52Z
dc.date.issued2015
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractThe antioxidant activities of the extracts prepared from Brassica napus's seeds (canola seeds) was determined in this study. Brassica napus's seeds were collected from eight different regions of Turkey. Extraction experiments were carried out with 100% hexane, 100% acetone, 100% methanol, 100% ethanol, % 75 hexane-water, % 75 acetone-water, 75% methanol-water, 75% ethanol-water, % 50 hexane-water, 50% acetone-water, 50% methanol-water, 50% ethanol-water at 25 degrees C overnight and were mixed by magnetic stirrer during 1h, 2h, 4h, 8h to determine optimal extraction conditions. According to the results of UV/VIS spectra, 100% hexane extraction managed at 25 degrees C overnight was chosen for working with ABTS/Persulphate, N, N-dimethyl-p-phenylenediamine (DMPD) and CUPRAC methods. CUPRAC and ABTS/Persulphate methods's total antioxidant capacity (TAC) were calculated as Trolox equivalent. In DMPD method, antioxidant capacity of the extracts was determined by inhibition of DMPD radical cation. DMPD method analysis results of Brassica napus's seeds showed the greatest effectiveness, with inhibition values of 2.00 +/- 0.07% and 17.1 +/- 0.03%. The antioxidant activities of Brassica napus's seeds ranged from 0.29 +/- 0.04 mol/g and 0.48 +/- 0.01 mol/g for CUPRAC method and 0.36 +/- 0.01 mol/g and 0.55 +/- 0.05 mol/g for ABTS/Persulphate method.
dc.identifier.endpage158
dc.identifier.issn1309-0801
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84931074645
dc.identifier.scopusqualityN/A
dc.identifier.startpage153
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7200
dc.identifier.volume19
dc.identifier.wosWOS:000361222900010
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorKarasakal, Ayça
dc.language.isoen
dc.publisherMarmara Univ, Fac Pharmacy
dc.relation.ispartofMarmara Pharmaceutical Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAntioxidant activity
dc.subjectBrassica napus's seed
dc.subjectABTS/Persulphate
dc.subjectCUPRAC
dc.subjectDMPD Methods
dc.subjectTrolox
dc.subjectPhenolic Composition
dc.subjectCapacity
dc.subjectCanola
dc.subjectTannins
dc.subjectTea
dc.titleEvaluation of Antioxidant activities of Brassica napus's seeds by CUPRAC, ABTS/Persulphate and DMPD methods
dc.typeArticle

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