The effects of Eicosapentaenoic acid on the endothelium of the carotid artery of rabbits on a high-cholesterol diet
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Dosyalar
Tarih
2010
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
F Hernandez
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The preventive and therapeutic effects of Eicosapentaenoic acid (EPA) on diet-induced hyperlipidemia in rabbits have been investigated. Eighteen New Zealand rabbits were randomly divided into three groups of 6 subjects each; experimental group-I (EG-I) was administered a cholesterol rich diet, experimental group-II (EG-II) was treated with EPA (300 mg/kg/d) following a cholesterol-rich diet and the control group (CG) had a standard diet. Blood samples were collected at day 0 and at the 4th and 12th weeks of EG-II to obtain serum levels of total cholesterol (TC), high density lipid-cholesterol (HDL-C), low density lipid-cholesterol (LDL-C) and triglyceride (TG). From each group tissue samples were collected from the carotid artery for immunohistochemistry and electron microscopy. Our results showed that EPA could significantly lower (p<0.001) serum TC, LDL-C, HDLC and TG levels with a reduction of 35%; 55%; 44% and 51%, respectively. Scanning and transmission electron microscopy results revealed that endothelial damage was more prominent in EG-I when compared to EG-II. The ruptured endothelial lining and damaged cellular surface was increased in EG-I when compared to EG-II. Ultrastructural observations showed that after EPA treatment, the degeneration and cellular surface damage on the endothelium were also decreased. These biochemical and ultrastructural results suggest that EPA is a potential drug which significantly lowers the serum lipid profile and partially repairs endothelial dysfunction due to hyperlipidemia.
Açıklama
Anahtar Kelimeler
EPA, Cholesterol, Atherosclerosis, Endothelium, Ultrastructure, Rabbit, All-Cis-5,8,11,14,17-Icosapentaenoate Epa-E, Polyunsaturated Fatty-Acids, Fish-Oil Supplementation, Blood-Pressure, Ethyl All-Cis-5,8,11,14,17-Icosapentaenoate, Plasma-Lipids, Docosahexaenoic Acid, Lipoproteins, Vasculogenesis, Angiogenesis
Kaynak
Histology and Histopathology
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
25
Sayı
2