Firmicutes/Bacteroidetes Ratio in the Gut Microbiota and IL-1?, IL-6, IL-8, TLR2, TLR4, TLR5 Gene Expressions in Type 2 Diabetes

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dc.authorscopusid57221970599
dc.authorscopusid57220204043
dc.authorscopusid56118245000
dc.authorscopusid6507755715
dc.authorscopusid57194164764
dc.authorscopusid57211552167
dc.authorscopusid6506930479
dc.contributor.authorBahar-Tokman, H.
dc.contributor.authorDemirci, Mehmet
dc.contributor.authorKeskin, Fatma E.
dc.contributor.authorÇağatay, P.
dc.contributor.authorTaner, Z.
dc.contributor.authorÖztürk-Bakar, Y.
dc.contributor.authorKiraz, Nuri
dc.contributor.authorKocazeybek, B.S.
dc.date.accessioned2023-04-20T08:04:16Z
dc.date.available2023-04-20T08:04:16Z
dc.date.issued2022
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalı
dc.description.abstractBackground: The aim of this study was to determine the Firmicutes/Bacteroidetes ratio in the gut microbiota and IL-1?, IL-6, IL-8, TLR2, TLR4 and TLR5 gene expression levels in the blood of adult type 2 diabetes (T2D) patients and compare it with that of adult nondiabetic healthy controls (HC). Methods: Between May 2016 and April 2017, 99 T2D patients and 99 HCs were enrolled in the study. Bacteroidetes and Firmicutes levels were assessed from stool sample DNA and IL-1?, IL-6, IL-8, TLR2, TLR4, and TLR5 gene expression levels assesed from blood sample RNA via qPCR from both T2D patients and healthy controls. Results: The Firmicutes/Bacteroidetes ratio detected in the stool of type 2 diabetes patients was found to be higher with a statistically significant difference (p < 0.0001). Gene expression levels of IL-1?, IL-6, IL-8, TLR2, TLR4, and TLR5 were found to be upregulated. Conclusions: The highest upregulation was detected in IL-6 with 11 fold in T2D patients comparing with HCs. F/B ratio and gene expression levels were elevated in T2D patients. Firmicutes were positively correlated with studied gene expressions. A better understanding of the complex interaction between gut microbiota, environment, and diabetes will allow for more effective prevention and treatment strategies for T2D. © 2022 Verlag Klinisches Labor GmbH. All rights reserved.
dc.description.sponsorshipIstanbul Üniversitesi: 3748, TSA-2016-3748
dc.description.sponsorshipWe are grateful for the support of Istanbul University. This research was supported by the Scientific Research Fund of Istanbul University and Istanbul University - Cerrahpasa, Project and Grant No.: 3748 (TSA-2016-3748).
dc.identifier.doi10.7754/Clin.Lab.2022.211244
dc.identifier.endpage1910
dc.identifier.issn1433-6510
dc.identifier.issue9en_US
dc.identifier.pmid36125161
dc.identifier.scopus2-s2.0-85138143372
dc.identifier.scopusqualityQ3
dc.identifier.startpage1903
dc.identifier.urihttps://doi.org/10.7754/Clin.Lab.2022.211244
dc.identifier.urihttps://hdl.handle.net/20.500.11776/11061
dc.identifier.volume68
dc.identifier.wosWOS:000920514000001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKiraz, Nuri
dc.language.isoen
dc.publisherVerlag Klinisches Labor GmbH
dc.relation.ispartofClinical Laboratory
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBacteroidetes
dc.subjectFirmicutes
dc.subjectGut microbiota
dc.subjectILs
dc.subjectTLRs
dc.subjectType 2 diabetes
dc.subjectinterleukin 1beta
dc.subjectinterleukin 6
dc.subjectinterleukin 8
dc.subjecttoll like receptor 2
dc.subjecttoll like receptor 4
dc.subjecttoll like receptor 5
dc.subjectinterleukin 6
dc.subjectinterleukin 8
dc.subjectRNA
dc.subjectTLR2 protein, human
dc.subjectTLR4 protein, human
dc.subjecttoll like receptor 2
dc.subjecttoll like receptor 4
dc.subjecttoll like receptor 5
dc.subjectadult
dc.subjectaged
dc.subjectArticle
dc.subjectBacteroidetes
dc.subjectblood sampling
dc.subjectcontrolled study
dc.subjectdiabetic patient
dc.subjectfeces analysis
dc.subjectfemale
dc.subjectFirmicutes
dc.subjectgene expression level
dc.subjecthuman
dc.subjectintestine flora
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectnon insulin dependent diabetes mellitus
dc.subjectreal time polymerase chain reaction
dc.subjectupregulation
dc.subjectBacteroidetes
dc.subjectFirmicutes
dc.subjectgene expression
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectAdult
dc.subjectBacteroidetes
dc.subjectDiabetes Mellitus, Type 2
dc.subjectFirmicutes
dc.subjectGastrointestinal Microbiome
dc.subjectGene Expression
dc.subjectHumans
dc.subjectInterleukin-6
dc.subjectInterleukin-8
dc.subjectRNA
dc.subjectToll-Like Receptor 2
dc.subjectToll-Like Receptor 4
dc.subjectToll-Like Receptor 5
dc.titleFirmicutes/Bacteroidetes Ratio in the Gut Microbiota and IL-1?, IL-6, IL-8, TLR2, TLR4, TLR5 Gene Expressions in Type 2 Diabetes
dc.typeArticle

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