Firmicutes/Bacteroidetes Ratio in the Gut Microbiota and IL-1?, IL-6, IL-8, TLR2, TLR4, TLR5 Gene Expressions in Type 2 Diabetes
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Verlag Klinisches Labor GmbH
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background: The aim of this study was to determine the Firmicutes/Bacteroidetes ratio in the gut microbiota and IL-1?, IL-6, IL-8, TLR2, TLR4 and TLR5 gene expression levels in the blood of adult type 2 diabetes (T2D) patients and compare it with that of adult nondiabetic healthy controls (HC). Methods: Between May 2016 and April 2017, 99 T2D patients and 99 HCs were enrolled in the study. Bacteroidetes and Firmicutes levels were assessed from stool sample DNA and IL-1?, IL-6, IL-8, TLR2, TLR4, and TLR5 gene expression levels assesed from blood sample RNA via qPCR from both T2D patients and healthy controls. Results: The Firmicutes/Bacteroidetes ratio detected in the stool of type 2 diabetes patients was found to be higher with a statistically significant difference (p < 0.0001). Gene expression levels of IL-1?, IL-6, IL-8, TLR2, TLR4, and TLR5 were found to be upregulated. Conclusions: The highest upregulation was detected in IL-6 with 11 fold in T2D patients comparing with HCs. F/B ratio and gene expression levels were elevated in T2D patients. Firmicutes were positively correlated with studied gene expressions. A better understanding of the complex interaction between gut microbiota, environment, and diabetes will allow for more effective prevention and treatment strategies for T2D. © 2022 Verlag Klinisches Labor GmbH. All rights reserved.
Açıklama
Anahtar Kelimeler
Bacteroidetes, Firmicutes, Gut microbiota, ILs, TLRs, Type 2 diabetes, interleukin 1beta, interleukin 6, interleukin 8, toll like receptor 2, toll like receptor 4, toll like receptor 5, interleukin 6, interleukin 8, RNA, TLR2 protein, human, TLR4 protein, human, toll like receptor 2, toll like receptor 4, toll like receptor 5, adult, aged, Article, Bacteroidetes, blood sampling, controlled study, diabetic patient, feces analysis, female, Firmicutes, gene expression level, human, intestine flora, major clinical study, male, non insulin dependent diabetes mellitus, real time polymerase chain reaction, upregulation, Bacteroidetes, Firmicutes, gene expression, genetics, metabolism, Adult, Bacteroidetes, Diabetes Mellitus, Type 2, Firmicutes, Gastrointestinal Microbiome, Gene Expression, Humans, Interleukin-6, Interleukin-8, RNA, Toll-Like Receptor 2, Toll-Like Receptor 4, Toll-Like Receptor 5
Kaynak
Clinical Laboratory
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
68
Sayı
9
