Molecular, serological and transmission electron microscopic analysis of the Barley yellow dwarf virus-PAV and the Cereal yellow dwarf virus-RPV in canary seed (Phalaris canariensis L.)

Basit Öğe Kaydı
dc.authorid0000-0002-0016-190X
dc.authorid0000-0002-2556-2478
dc.authorid0000-0002-2556-2478
dc.authorid0000-0002-2556-2478
dc.authorscopusid8869167100
dc.authorscopusid6603225066
dc.authorscopusid6508312772
dc.authorscopusid7004323565
dc.authorscopusid8869166900
dc.authorscopusid22949946700
dc.authorscopusid8681535200
dc.authorwosidILBAGI, Havva/ABA-4459-2020
dc.authorwosidBudak, Hikmet/F-4708-2010
dc.authorwosidBudak, Hikmet/S-5835-2017
dc.authorwosidBudak, Hikmet/U-8517-2019
dc.contributor.authorİlbağı, Havva
dc.contributor.authorRabenstein, F.
dc.contributor.authorHabekuss, A.
dc.contributor.authorOrdon, F.
dc.contributor.authorÇıtır, Ahmet
dc.contributor.authorCebeci, O.
dc.contributor.authorBudak, H.
dc.date.accessioned2022-05-11T14:43:26Z
dc.date.available2022-05-11T14:43:26Z
dc.date.issued2008
dc.departmentFakülteler, Ziraat Fakültesi, Bitki Koruma Bölümü
dc.description.abstractCanary seed (Phalaris canariensis L.) is a cereal crop belonging to the tribe Phalarideae of the Poaceae family. A prevailing virus infection, which causes dwarfing of plants and yellowing of leaves, was observed in canary seed fields in the Tekirdag province of Turkey. The aim of this study was to identify, clone and sequence the cereal viruses naturally occurring on P. canariensis by employing serological tests as DAS-ELISA and TAS-ELISA tests combined with transmission electron microcopy (TEM) and molecular analysis. One hundred and one plant samples showing symptoms were collected and tested serologically using polyclonal antisera against Barley yellow dwarf virus-PAV (BYDV-PAV) and Cereal yellow dwarf virus-RPV (CYDV-RPV). The results of both immunoassays showed that 48% of the samples were infected with BYDV-PAV, 2% with CYDV-RPV and 14% were mixed infection. 36% of the samples were uninfected or infected at level below detection. Aphid transmission experiments revealed that barley (cv. Rubina) exhibited characteristic of CYDV-RPV. Investigations of infected canary grass seedlings using transmission electron microscopy (TEM) supported the findings of serological tests and revealed the presence of isometric particles of approximately 25 nm in diameter. These results were also confirmed by using BYDV-PAV and CYDV-RPV specific primers. Sequence analysis of cDNA and probable translation products revealed a high level of homology to BYDV-PAV and CYDV-RPV isolates found in other plant species. The sequence data obtained from this research were deposited in the EMBL/GenBank Data Libraries under the accession nos. EGO19056 and EF372272.
dc.identifier.doi10.1556/CRC.36.2008.2.3
dc.identifier.endpage234
dc.identifier.issn0133-3720
dc.identifier.issn1788-9170
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-52649113724
dc.identifier.scopusqualityQ2
dc.identifier.startpage225
dc.identifier.urihttps://doi.org/10.1556/CRC.36.2008.2.3
dc.identifier.urihttps://hdl.handle.net/20.500.11776/9603
dc.identifier.volume36
dc.identifier.wosWOS:000256573100003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorİlbağı, Havva
dc.institutionauthorÇıtır, Ahmet
dc.language.isoen
dc.publisherSpringer Heidelberg
dc.relation.ispartofCereal Research Communications
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectcereals viruses
dc.subjectcDNA
dc.subjectDAS-ELISA
dc.subjectTAS-ELISA
dc.subjectVector Relationships
dc.subjectTrakya Region
dc.titleMolecular, serological and transmission electron microscopic analysis of the Barley yellow dwarf virus-PAV and the Cereal yellow dwarf virus-RPV in canary seed (Phalaris canariensis L.)
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
9603.pdf
Boyut:
434.36 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Ziraat Fakültesi Koleksiyonu