Effects of combined administration of doxorubicin and chloroquine on lung pathology in mice with solid Ehrlich ascites carcinoma

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dc.authorid0000-0001-8256-9943
dc.authorscopusid23111808300
dc.authorscopusid57115408300
dc.authorscopusid6505825773
dc.authorscopusid57197854869
dc.authorscopusid14830362700
dc.authorscopusid57185264300
dc.authorscopusid23975103000
dc.authorwosidSönmez, Kıvılcım/D-4857-2019
dc.contributor.authorUtkusavaş, Ayfer
dc.contributor.authorGürel Gürevin, Ebru
dc.contributor.authorYılmazer, Nadim
dc.contributor.authorÜvez, Ayca
dc.contributor.authorÖztay, Füsun
dc.contributor.authorBulut, Huri
dc.contributor.authorArmutak, Elif İlkay
dc.date.accessioned2022-05-11T14:03:15Z
dc.date.available2022-05-11T14:03:15Z
dc.date.issued2022
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractCombined use of a chemotherapeutic agent and an autophagy inhibitor is a novel cancer treatment strategy. We investigated the effects of chloroquine (CQ) on lung pathology caused by both solid Ehrlich ascites carcinoma (EAC) and doxorubicin (DXR). A control group and eight experimental groups of adult female mice were inoculated subcutaneously with 2.5 x 10(6) EAC cells. DXR (1.5 mg/kg and 3 mg/kg) and CQ (25 mg/kg and 50 mg/kg) alone or in combination were injected intraperitoneally on days 2, 7 and 12 following inoculation with EAC cells. Lung tissue samples were examined using immunohistochemistry (IHC) for endothelial (eNOS), inducible nitric oxide synthase (iNOS) and neutrophil gelatinase-associated lipocalin (NGAL). Serum catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) and malondialdehyde (MDA) levels were measured using ELISA. We found decreased levels of iNOS and eNOS in the groups that received 1.5 mg/kg DXR alone and in combination with 25 mg/kg and 50 mg/kg CQ. Combined administration of DXR and CQ partially prevented disruption of alveolar structure. Levels of antioxidant enzymes and MDA were lower in all treated groups; the greatest reduction was observed in mice that received the combination of 25 mg/kg CQ + 1.5 mg/kg DXR. Levels of NGAL were elevated in all treated groups. We found that CQ ameliorated both EAC and DOX induced lung pathology in female mice with solid EAC by reducing oxidative stress.
dc.identifier.doi10.1080/10520295.2022.2036369
dc.identifier.issn1052-0295
dc.identifier.issn1473-7760
dc.identifier.pmid35240890
dc.identifier.scopus2-s2.0-85126021989
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1080/10520295.2022.2036369
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4650
dc.identifier.wosWOS:000764954500001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorYılmazer, Nadim
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofBiotechnic & Histochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectChloroquine
dc.subjectdoxorubicin
dc.subjectEhrlich ascites carcinoma
dc.subjectlung
dc.subjectmice
dc.subjectoxidative stress
dc.subjectGelatinase-Associated Lipocalin
dc.subjectOxidative Stress
dc.subjectNitric-Oxide
dc.subjectCancer-Cells
dc.subjectNo Synthase
dc.subjectRat-Liver
dc.subjectApoptosis
dc.subjectAutophagy
dc.subjectNgal
dc.subjectInhibition
dc.titleEffects of combined administration of doxorubicin and chloroquine on lung pathology in mice with solid Ehrlich ascites carcinoma
dc.typeArticle

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