Functional and Biochemical Properties of Proteins from Safflower Seed

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dc.authorscopusid6603077608
dc.authorscopusid23101201700
dc.authorscopusid26538970400
dc.authorwosidÖZCAN, Hakkı/AAJ-6857-2021
dc.contributor.authorSağıroğlu, Ayten
dc.contributor.authorÖzcan, Hakkı Mevlüt
dc.contributor.authorŞatana, Aziz
dc.date.accessioned2022-05-11T14:47:00Z
dc.date.available2022-05-11T14:47:00Z
dc.date.issued2009
dc.departmentMeslek Yüksekokulları, Hayrabolu Meslek Yüksekokulu, Bitkisel ve Hayvansal Üretim Bölümü
dc.description.abstractThere are relatively few studies on the properties of proteins that comprise a major part of the safflower seed. The biochemical and functional properties of these proteins have not been fully discovered. In this study, safflower seed proteins were obtained by isoelectric precipitation in two fractions. One of the fractions (Fraction-1) was obtained at pH 10 and the other fraction was obtained, as the protein supernatant separate from the pH precipitate, by ultra filtration (Fraction-2). Functional and biochemical properties of both of fractions were investigated. Gel permeation chromatography (GPC) was applied to both fractions. GPC shows that high-molecular weight constituents are present only in fraction 1, whereas fraction 2 consists of proteins with lower molecular weights in comparison with protein standards. Sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) electrophoresis in the presence of mercaptoethanol and SDS with protein weight markers was applied to both of the fractions. The proteins in both of the fractions were separated and stained with Commassie Brillant blue R-250 dye on the PAGE gel. The molecular weight (Mw) of each protein band was determined graphically by plotting Log Mw and relative mobilities (Rf) using GS-300 scanning densitometry and a suitable computer program.
dc.identifier.doi10.1080/10826060902800833
dc.identifier.endpage169
dc.identifier.issn1082-6068
dc.identifier.issn1532-2297
dc.identifier.issue2en_US
dc.identifier.pmid19291578
dc.identifier.scopus2-s2.0-65749118644
dc.identifier.scopusqualityQ3
dc.identifier.startpage159
dc.identifier.urihttps://doi.org/10.1080/10826060902800833
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10412
dc.identifier.volume39
dc.identifier.wosWOS:000264224600006
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorŞatana, Aziz
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.ispartofPreparative Biochemistry & Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBiochemical and functional properties
dc.subjectSafflower seed (Carthamus tintorius L
dc.subject)
dc.subjectSeed protein
dc.titleFunctional and Biochemical Properties of Proteins from Safflower Seed
dc.typeArticle

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