Micropropagation of Garlic Chives (Allium Tuberosum Rottl. Ex Sprang) Using Mesocotyl Axis

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dc.authorid0000-0001-5110-6014
dc.authorid0000-0001-5127-1482
dc.authorwosidÖzcan, Sebahattin/AAF-6031-2020
dc.authorwosidKhawar, Khalid Mahmood/C-1469-2009
dc.contributor.authorAlizadeh, B.
dc.contributor.authorDaneshvar Royandazagh, Sheida
dc.contributor.authorKhawar, K. M.
dc.contributor.authorÖzcan, Seçkin
dc.date.accessioned2022-05-11T14:45:22Z
dc.date.available2022-05-11T14:45:22Z
dc.date.issued2013
dc.departmentFakülteler, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü
dc.description.abstractGarlic chives - Allium tuberosum Rottl. ex Sprang is widely distributed from South East Asia to the Middle East. They are grown for their garlic flavored leaves and are used for culinary purposes. The plants are apomictic, which makes creation of variations and development of new varieties very difficult. The results of previous research show recalcitrant regeneration behaviour in in vitro micropropagation cultures. The study was designed to develop efficient regeneration protocols that could help in easy genetic transformation and mutation studies on MS medium containing various concentrations of TDZ-NAA, TDZ-2, 4-D and BAP-IBA using juvenile mesocotyl axis explant obtained from of one week old in vitro grown seedlings. The concentrations and combinations of different plant growth regulators in MS medium had significantly different and variable effects on bulblet regeneration. Maximum number of 7.20 shoots per explant was recorded on MS medium containing 1 mg/l BAP-2 mg/l IBA. The shoots regenerated on any regeneration medium were not difficult to root on MS medium containing 0.5 mg/l IBA. These rooted plants were easily acclimatized in the growth chamber. This study clearly shows that mesocotyl axis explants of garlic chives can be successfully cultured on BAP-IBA containing medium to regenerate axillary bulblets. To conclude, this protocol provides a successful and reliable propagation technique to regenerate garlic chive coleoptile axis excised from of one week old in vitro regenerated seedlings for the first time. This would help in advanced genetic transformation and mutation studies of the plant in future.
dc.identifier.endpage549
dc.identifier.issn1018-7081
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84877248772
dc.identifier.scopusqualityQ4
dc.identifier.startpage543
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10022
dc.identifier.volume23
dc.identifier.wosWOS:000318671300034
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorDaneshvar Royandazagh, Sheida
dc.language.isoen
dc.publisherPakistan Agricultural Scientists Forum
dc.relation.ispartofJournal of Animal and Plant Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectGarlic chive
dc.subjectmesocotyl axis
dc.subjectin vitro
dc.subjectmicropropagation
dc.subjectrooting
dc.subjectacclimatization
dc.subjectIn-Vitro Propagation
dc.subjectTwin Scale Explants
dc.subjectPlant-Regeneration
dc.subjectChinese Chive
dc.subjectSomatic Embryogenesis
dc.subjectSuspension-Cultures
dc.subjectBulblet Formation
dc.subjectCallus Formation
dc.subjectTissue-Culture
dc.subjectBarley
dc.titleMicropropagation of Garlic Chives (Allium Tuberosum Rottl. Ex Sprang) Using Mesocotyl Axis
dc.typeArticle

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