Genetic variation of insulin-like growth factor II (IGF-II) gene and its associations with growth traits in european sea bass (dicentrarchus labrax)
Yükleniyor...
Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Central Fisheries Research Inst
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Insulin-like growth factor II (IGF-II) is a peptide hormone which plays a crucial role in the growth and metabolic regulations of vertebrates. In this study, we identified single nucleotide polymorphisms on IGF-II gene in the European sea bass (Dicentrarchus labrax) and revealed the potential association between the genotypes of IGF-II gene and growth traits in 150 European sea bass. The polymorphisms were determined using DNA sequencing and PCR-RFLP methods in two different regions of IGF-II gene. An AT indel and two SNPs as g.5127711C>G and g.5127731G>T in the intron 2 region, and one SNP as g.5129188C>T in the intron 3 region of IGF-II gene in the European sea bass were reported for the first time with this study. Two different genotypes (GG, TG) were observed and the G allele was predominant (65%) in the IGF-II-NdeI locus. Associations between the genotypes of IGF-II-NdeI locus and body weight and total length were statistically significant (P<0.05). Body weight and total length in each group were higher for GG genotype of IGF-II-NdeI locus (p<0.05). In conclusion, the IGF-II-NdeI locus had a significant effect on the body weight and total length which could be useful for sea bass selection and breeding for marker-assisted selection program. © 2020, Central Fisheries Research Inst. All rights reserved.
Açıklama
Anahtar Kelimeler
Dicentrarchus labrax, Gene, Growth Traits, IGF-II, SNP
Kaynak
Turkish Journal of Fisheries and Aquatic Sciences
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
20
Sayı
7
