Does leukocyte-poor or leukocyte-rich platelet-rich plasma applied with biopolymers have superiority to conventional platelet-rich plasma applications on chondrocyte proliferation?

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dc.authorid0000-0003-2003-6337
dc.authorscopusid56769801000
dc.authorscopusid35197435300
dc.authorscopusid56258081800
dc.authorscopusid56891784100
dc.authorscopusid56002899200
dc.authorwosidsirin, duygu yasar/AAR-8685-2020
dc.authorwosidYILMAZ, Ibrahim/H-6199-2019
dc.authorwosidMahirogullari, Mahir/AAA-4742-2020
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorİşyar, Mehmet
dc.contributor.authorÖznam, Kadir
dc.contributor.authorMahiroğulları, Mahir
dc.date.accessioned2022-05-11T14:28:36Z
dc.date.available2022-05-11T14:28:36Z
dc.date.issued2017
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractObjectives: This study aims to investigate the possible effects of leukocyte concentration in the content of platelet-rich plasma (PRP) and the administration of PRP using a drug delivery system on chondrocyte proliferation in vitro conditions. Patients and methods: Blood from nine male patients (mean age 65 years; range 49 to 81 years) with advanced stage osteoarthritis who had not responded to medical or conservative treatments and underwent total knee arthroplasty was used to prepare two formulations: PRP with low concentration leukocytes (2000-4000 leukocytes/mu L) was designated as pure PRP (P-PRP), whereas PRP with high concentration leukocytes (9000-11000 leukocytes/mu L) as leukocyte-rich PRP (L-PRP). Samples were divided into five groups as control group (group 1), chondrocyte cultures with P-PRP applied directly (group 2), chondrocyte cultures with L-PRP applied directly (group 3), chondrocytes co-cultured with P-PRP applied hydrogel (group 4), and chondrocytes co-cultured with L-PRP applied hydrogel (group 5). In all groups; cell morphology, viability and proliferation were compared with the expression of stage-specific embryonic antigen-1 (SSEA-1), a precondrocyte marker. Results: Maximum cell proliferation and SSEA-1 expression occurred in group 4, with a statistically significant correlation between SSEA-1 expression and cell proliferation. Conclusion: Our study showed the importance of leukocyte concentration of PRP and efficiency of delivery systems such as hydrogel and that L-PRP administered with a delivery system is more efficient than conventional applications of PRP in the treatment of cartilage damage.
dc.description.sponsorshipNamik Kemal University Scientific Research Administration Division [NKUBAP.00.10.AR.15.08]
dc.description.sponsorshipThis study was supported by grants (Project no: NKUBAP.00.10.AR.15.08) from Namik Kemal University Scientific Research Administration Division.
dc.identifier.doi10.5606/ehc.2017.55186
dc.identifier.endpage151
dc.identifier.issn1305-8282
dc.identifier.issn1309-0313
dc.identifier.issue3en_US
dc.identifier.pmid29125811
dc.identifier.scopus2-s2.0-85034021288
dc.identifier.scopusqualityN/A
dc.identifier.startpage142
dc.identifier.urihttps://doi.org/10.5606/ehc.2017.55186
dc.identifier.urihttps://hdl.handle.net/20.500.11776/6887
dc.identifier.volume28
dc.identifier.wosWOS:000415126000002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorŞirin, Duygu Yaşar
dc.language.isoen
dc.publisherTurkish Joint Diseases Foundation
dc.relation.ispartofEklem Hastaliklari Ve Cerrahisi-Joint Diseases and Related Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCo-culture techniques
dc.subjecthydrogel
dc.subjectplatelet-rich plasma
dc.subjectstage-specific embryonic antigens
dc.subjectCartilage
dc.subjectFormulations
dc.subjectTherapy
dc.subjectRepair
dc.subjectCells
dc.subjectBone
dc.titleDoes leukocyte-poor or leukocyte-rich platelet-rich plasma applied with biopolymers have superiority to conventional platelet-rich plasma applications on chondrocyte proliferation?
dc.typeArticle

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