Nigella sativa attenuates bleomycin-induced pulmonary fibrosis in rats by inhibition of inflammation, fibrosis, and inducible nitric oxide synthase

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dc.authorscopusid36023238400
dc.authorscopusid56816914100
dc.authorscopusid55134339900
dc.authorscopusid24436194200
dc.authorscopusid7006356462
dc.contributor.authorErboğa, Mustafa
dc.contributor.authorErboğa, Zeynep Fidanol
dc.contributor.authorDönmez, Yeliz Bozdemir
dc.contributor.authorAktaş, Cevat
dc.contributor.authorKanter, Mehmet
dc.date.accessioned2022-05-11T14:41:28Z
dc.date.available2022-05-11T14:41:28Z
dc.date.issued2015
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.description.abstractThe present study investigated the anti-oxidant, anti-inflammatory and anti-fibrotic potential of Nigella sativa (NS) against bleomycin (BLC)-induced lung injury and fibrosis, an experimental animal model. A total of 18 male Sprague-Dawley rats were divided into three groups: Control, BLC, BLC+NS; each group consist of 6 animals. Pulmonary fibrosis was induced by a single intratracheal instillation of 2.5 mg/kg BLC. BLC+NS group rats were intragastric administered daily 400 mg/kg of NS from day 1 to 28. At the end of the study, lung tissues were removed for histopathological and immunohistochemical investigation. Lung tissues were stained with hematoxylin and eosin and Masson's Trichrome for histological evaluation. Moreover, the inducible nitric oxide synthase (iNOS) expression in the lung tissues was determined by immunohistochemical staining. BLC-induced histological changes including lung inflammation and lung fibrosis were significantly detected compared to the control group. NS treatment significantly ameliorated the BLC mediated histological changes and reduced the inflammatory cell infiltrate in lung tissues. NS significantly blocked collagen accumulations with parallel reduction in the fibrosis score. In addition, NS also markedly decreased the positive staining of iNOS in lung tissues. Our study provides evidence that NS significantly ameliorated BLM-induced pulmonary fibrosis in rats via the inhibition of inflammation score, fibrosis score, and iNOS expression. Therefore, NS may be a potential therapeutic reagent for the treatment of lung fibrosis. © 2015 OMU.
dc.identifier.doi10.5835/jecm.omu.32.03.005
dc.identifier.endpage125
dc.identifier.issn1309-4483
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85006683745
dc.identifier.scopusqualityQ4
dc.identifier.startpage121
dc.identifier.urihttps://doi.org/10.5835/jecm.omu.32.03.005
dc.identifier.urihttps://hdl.handle.net/20.500.11776/9189
dc.identifier.volume32
dc.indekslendigikaynakScopus
dc.institutionauthorErboğa, Mustafa
dc.institutionauthorErboğa, Zeynep Fidanol
dc.institutionauthorDönmez, Yeliz Bozdemir
dc.institutionauthorAktaş, C.
dc.language.isoen
dc.publisherOndokuz Mayis Universitesi
dc.relation.ispartofJournal of Experimental and Clinical Medicine (Turkey)
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBleomycin
dc.subjectInducible nitric oxide synthase
dc.subjectNigella sativa
dc.subjectPulmonary fibrosis
dc.subjectRat
dc.subjectcollagen
dc.subjectinducible nitric oxide synthase
dc.subjectNigella sativa extract
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectantiinflammatory activity
dc.subjectArticle
dc.subjectbleomycin-induced pulmonary fibrosis
dc.subjectcell infiltration
dc.subjectcontrolled study
dc.subjecthistopathology
dc.subjectimmunohistochemistry
dc.subjectinflammatory cell
dc.subjectlung fibrosis
dc.subjectlung infiltrate
dc.subjectlung parenchyma
dc.subjectmale
dc.subjectnonhuman
dc.subjectpneumonia
dc.subjectrat
dc.titleNigella sativa attenuates bleomycin-induced pulmonary fibrosis in rats by inhibition of inflammation, fibrosis, and inducible nitric oxide synthase
dc.typeArticle

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