Effects of calcium hydroxide and N-acetylcysteine on MMP-2, MMP-9, TIMP-1 and TIMP-2 in LPS-stimulated macrophage cell lines

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dc.authorid0000-0003-1811-3858
dc.authorid0000-0002-1457-4721
dc.authorscopusid57188740173
dc.authorscopusid56092758200
dc.authorscopusid7003894097
dc.authorscopusid57091615400
dc.authorscopusid57212894389
dc.authorscopusid8235840100
dc.authorwosidAKKAYA, ZULFIYE YELIZ/I-8281-2013
dc.authorwosidCEYHAN, DENIZ/J-1527-2013
dc.authorwosidceyhan, deniz/AAN-6242-2021
dc.authorwosidPEYNIRCIOGLU, BANU/I-8337-2013
dc.contributor.authorAstantas, Eda Ezgi
dc.contributor.authorAksoy, Yasemin
dc.contributor.authorUlum, Yeliz Zulfiye Akkaya
dc.contributor.authorCeyhan, Deniz
dc.contributor.authorPeynircioglu, Banu
dc.contributor.authorBuzoğlu, Hatice Doğan
dc.date.accessioned2022-05-11T14:30:59Z
dc.date.available2022-05-11T14:30:59Z
dc.date.issued2018
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractAim: This study was evaluated the effects of N-acetylcysteine (NAC) and calcium hydroxide (Ca(OH)(2)) on the expression levels of matrix metalloproteinase -2, -9 (MMP-2, -9) and tissue inhibitor metalloproteinase -1, -2 (TIMP-1, -2) in lipopolysaccharide (LPS)-stimulated human macrophages. Methods: Human monocyte precursor cells (THP-1) were differentiated into macrophage-adherent cells and were stimulated with LPS for 24 h. Then individually incubated with NAC or Ca(OH)(2) for 24, 48 and 72 h. Following incubation, protein expression and mRNA levels of MMP-2, -9 and TIMP-1, -2 were evaluated using enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRT-PCR). Data were statistically analysed using two-way ANOVA, to followed by Bonferroni test at alpha=0.05. Results: NAC significantly decreased mRNA expression and protein levels of MMP-9, while Ca(OH)(2) decreased mRNA expression alone at 24 h. NAC and Ca(OH)2 decreased mRNA expression of MMP-2 at 24 h, while NAC increased this expression at 48 h. Although NAC and Ca(OH)2 decreased the mRNA expression of TIMP-1, -2 at 24 h, only NAC increased mRNA expression of TIMP-1 at 48 h. Conclusion: At the early stages of inflammation, NAC and Ca(OH)(2) have anti-inflammatory effects on macrophages.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [112S004]
dc.description.sponsorshipThis study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) (Project number: 112S004).
dc.identifier.doi10.1515/tjb-2017-0046
dc.identifier.endpage577
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85039057692
dc.identifier.scopusqualityQ4
dc.identifier.startpage571
dc.identifier.urihttps://doi.org/10.1515/tjb-2017-0046
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7263
dc.identifier.volume43
dc.identifier.wosWOS:000450015000001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorCeyhan, Deniz
dc.language.isoen
dc.publisherWalter De Gruyter Gmbh
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectNAC
dc.subjectCa(OH)(2)
dc.subjectMMP-2
dc.subjectMMP-9
dc.subjectTIMP-1
dc.subjectTIMP-2
dc.subjectMacrophage cell line
dc.subjectMatrix Metalloproteinases
dc.subjectPrevotella-Nigrescens
dc.subjectApical Periodontitis
dc.subjectPeriapical Lesions
dc.subjectTnf-Alpha
dc.subjectAntioxidant
dc.subjectActivation
dc.subjectExpression
dc.subjectDecreases
dc.subjectGrowth
dc.titleEffects of calcium hydroxide and N-acetylcysteine on MMP-2, MMP-9, TIMP-1 and TIMP-2 in LPS-stimulated macrophage cell lines
dc.typeArticle

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