The fate of 35S rRNA genes in the allotetraploid grass Brachypodium hybridum

Basit Öğe Kaydı
dc.authorscopusid57191574880
dc.authorscopusid7004490346
dc.authorscopusid56979278500
dc.authorscopusid57225437022
dc.authorscopusid57189634048
dc.authorscopusid56371114900
dc.authorscopusid57205373877
dc.contributor.authorBorowska-Zuchowska, N.
dc.contributor.authorKovarik, A.
dc.contributor.authorRobaszkiewicz, E.
dc.contributor.authorTuna, Metin
dc.contributor.authorTuna, G.S.
dc.contributor.authorGordon, S.
dc.contributor.authorHasterok, R.
dc.date.accessioned2022-05-11T14:07:14Z
dc.date.available2022-05-11T14:07:14Z
dc.date.issued2020
dc.departmentFakülteler, Ziraat Fakültesi, Tarla Bitkileri Bölümü
dc.description.abstractNucleolar dominance (ND) consists of the reversible silencing of 35S/45S rDNA loci inherited from one of the ancestors of an allopolyploid. The molecular mechanisms by which one ancestral rDNA set is selected for silencing remain unclear. We applied a combination of molecular (Southern blot hybridization and reverse-transcription cleaved amplified polymorphic sequence analysis), genomic (analysis of variants) and cytogenetic (fluorescence in situ hybridization) approaches to study the structure, expression and epigenetic landscape of 35S rDNA in an allotetraploid grass that exhibits ND, Brachypodium hybridum (genome composition DDSS), and its putative progenitors, Brachypodium distachyon (DD) and Brachypodium stacei (SS). In progenitor genomes, B. stacei showed a higher intragenomic heterogeneity of rDNA compared with B. distachyon. In all studied accessions of B. hybridum, there was a reduction in the copy number of S homoeologues, which was accompanied by their inactive transcriptional status. The involvement of DNA methylation in CG and CHG contexts in the silencing of the S-genome rDNA loci was revealed. In the B. hybridum allotetraploid, ND is stabilized towards the D-genome units, irrespective of the polyphyletic origin of the species, and does not seem to be influenced by homoeologous 35S rDNA ratios and developmental stage. © 2020 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.
dc.description.sponsorshipU.S. Department of Energy, USDOE: DE?AC02?05CH11231; Office of Science, SC; Grantová Agentura ?eské Republiky, GA ?R: 20-14133J; Narodowe Centrum Nauki, NCN: 2018/02/X/NZ3/03518, 2018/31/B/NZ3/01761
dc.description.sponsorshipWe thank Dr Glyn Jenkins (Aberystwyth University, UK) for his valuable comments on the article. The authors gratefully acknowledge financial support from the National Science Centre, Poland (grant no. 2018/31/B/NZ3/01761 and 2018/02/X/NZ3/03518) and the Czech Science Foundation (20-14133J). The work conducted by the US DOE Joint Genome Institute is supported by the Office of Science of the US Department of Energy under contract no. DE-AC02-05CH11231.
dc.description.sponsorshipWe thank Dr Glyn Jenkins (Aberystwyth University, UK) for his valuable comments on the article. The authors gratefully acknowledge financial support from the National Science Centre, Poland (grant no. 2018/31/B/NZ3/01761 and 2018/02/X/NZ3/03518) and the Czech Science Foundation (20?14133J). The work conducted by the US DOE Joint Genome Institute is supported by the Office of Science of the US Department of Energy under contract no. DE?AC02?05CH11231.
dc.identifier.doi10.1111/tpj.14869
dc.identifier.endpage1825
dc.identifier.issn0960-7412
dc.identifier.issue5en_US
dc.identifier.pmid32506573
dc.identifier.scopus2-s2.0-85087457230
dc.identifier.scopusqualityQ1
dc.identifier.startpage1810
dc.identifier.urihttps://doi.org/10.1111/tpj.14869
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5019
dc.identifier.volume103
dc.identifier.wosWOS:000545082000001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorTuna, Metin
dc.language.isoen
dc.publisherBlackwell Publishing Ltd
dc.relation.ispartofPlant Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject35S rDNA evolution
dc.subject35S rRNA gene expression
dc.subjectallopolyploidy
dc.subjectBrachypodium hybridum
dc.subjectnucleolar dominance
dc.subjectAlkylation
dc.subjectFluorescence microscopy
dc.subjectPlants (botany)
dc.subjectRNA
dc.subjectCleaved amplified polymorphic sequences
dc.subjectDevelopmental stage
dc.subjectFluorescence in situ hybridization
dc.subjectMolecular mechanism
dc.subjectNucleolar dominances
dc.subjectPolyphyletic origin
dc.subjectReverse transcription
dc.subjectSouthern blot hybridization
dc.subjectTranscription
dc.subjectAlkylation
dc.subjectGenes
dc.subjectHybridization
dc.subjectNucleic Acids
dc.subjectRatios
dc.subjectReduction
dc.subjectSet
dc.subjectBrachypodium
dc.subjectcopy number variation
dc.subjectDNA methylation
dc.subjectgene locus
dc.subjectgenetic polymorphism
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectmolecular evolution
dc.subjectplant chromosome
dc.subjectplant gene
dc.subjectplant genome
dc.subjectRNA gene
dc.subjectSouthern blotting
dc.subjecttetraploidy
dc.subjectBlotting, Southern
dc.subjectBrachypodium
dc.subjectChromosomes, Plant
dc.subjectDNA Copy Number Variations
dc.subjectDNA Methylation
dc.subjectEvolution, Molecular
dc.subjectGenes, Plant
dc.subjectGenes, rRNA
dc.subjectGenetic Loci
dc.subjectGenome, Plant
dc.subjectPolymorphism, Genetic
dc.subjectTetraploidy
dc.titleThe fate of 35S rRNA genes in the allotetraploid grass Brachypodium hybridum
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
5019.pdf
Boyut:
1.29 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Ziraat Fakültesi Koleksiyonu