Novel Tick Phlebovirus Genotypes Lacking Evidence for Vertebrate Infections in Anatolia and Thrace, Turkey

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dc.authorid0000-0001-5422-1982
dc.authorid0000-0001-5008-9443
dc.authorid0000-0002-4779-0893
dc.authorid0000-0002-5493-0807
dc.authorid0000-0001-8185-3176
dc.authorid0000-0002-1392-4048
dc.authorscopusid57209228994
dc.authorscopusid15769247000
dc.authorscopusid26326680200
dc.authorscopusid55258915300
dc.authorscopusid52263886700
dc.authorscopusid57193233348
dc.authorscopusid36439587600
dc.authorwosidŞAHAN, Adem/AAE-4282-2021
dc.authorwosidLinton, Yvonne-Marie/AAW-3992-2021
dc.authorwosidOzkul, Aykut/A-1973-2016
dc.authorwosidErgunay, Koray/I-8368-2013
dc.authorwosidLinton, Yvonne/AAG-2353-2019
dc.authorwosidhacioglu, sabri/ABG-9827-2021
dc.contributor.authorEmanet, Nergis
dc.contributor.authorKar, Sırrı
dc.contributor.authorDinçer, Ender
dc.contributor.authorBrinkmann, Annika
dc.contributor.authorHacioglu, Sabri
dc.contributor.authorFarzani, Touraj Aligholipour
dc.contributor.authorErgünay, Koray
dc.date.accessioned2022-05-11T14:28:40Z
dc.date.available2022-05-11T14:28:40Z
dc.date.issued2019
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractWe screened ticks and human clinical specimens to detect and characterize tick phleboviruses and pathogenicity in vertebrates. Ticks were collected at locations in Istanbul (Northwest Anatolia, Thrace), Edirne, Kirklareli, and Tekirdag (Thrace), Mersin (Mediterranean Anatolia), Adiyaman and Sanliurfa (Southeastern Anatolia) provinces from 2013-2018 and were analyzed following morphological identification and pooling. Specimens from individuals with febrile disease or meningoencephalitic symptoms of an unknown etiology were also evaluated. The pools were screened via generic tick phlebovirus amplification assays and products were sequenced. Selected pools were used for cell culture and suckling mice inoculations and next generation sequencing (NGS). A total of 7492 ticks were screened in 609 pools where 4.2% were positive. A phylogenetic sequence clustering according to tick species was observed. No human samples were positive. NGS provided near-complete viral replicase coding sequences in three pools. A comprehensive analysis revealed three distinct, monophyletic virus genotypes, comprised of previously-described viruses from Anatolia and the Balkans, with unique fingerprints in conserved amino acid motifs in viral replicase. A novel tick phlebovirus group was discovered circulating in the Balkans and Turkey, with at least three genotypes or species. No evidence for replication in vertebrates or infections in clinical cases could be demonstrated.
dc.description.sponsorshipArmed Forces Health Surveillance Board, Global Emerging Infections Surveillance and Response System (AFHSB-GEIS), United States of America [P0034_18_WR]; US ArmyUnited States Department of Defense [W911QY-16-C-0160]
dc.description.sponsorshipThe study was supported in part by the Armed Forces Health Surveillance Board, Global Emerging Infections Surveillance and Response System (AFHSB-GEIS), United States of America (FY18 award P0034_18_WR (PI: Yvonne-Marie Linton) under US Army subcontract W911QY-16-C-0160).
dc.identifier.doi10.3390/v11080703
dc.identifier.issn1999-4915
dc.identifier.issue8en_US
dc.identifier.pmid31374842
dc.identifier.scopus2-s2.0-85070452583
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3390/v11080703
dc.identifier.urihttps://hdl.handle.net/20.500.11776/6906
dc.identifier.volume11
dc.identifier.wosWOS:000482993900013
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKar, Sırrı
dc.language.isoen
dc.publisherMdpi
dc.relation.ispartofViruses-Basel
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjecttick
dc.subjectphlebovirus
dc.subjectspecies
dc.subjectgenotype
dc.subjectbunyavirus
dc.subjectTurkey
dc.subjectThrombocytopenia Syndrome
dc.subjectSevere Fever
dc.subjectBorne Phleboviruses
dc.subjectVirus
dc.subjectSequence
dc.subjectIdentification
dc.subjectAlignment
dc.subjectHyalomma
dc.titleNovel Tick Phlebovirus Genotypes Lacking Evidence for Vertebrate Infections in Anatolia and Thrace, Turkey
dc.typeArticle

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