Pharmaco-molecular assessment of the effects of anandamide and its antagonists on hippocampal tissue in Wistar albino rats

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dc.authorid0000-0003-2003-6337
dc.authorid0000-0002-1224-442X
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorÖzbek, Hanefi
dc.date.accessioned2022-05-11T14:12:15Z
dc.date.available2022-05-11T14:12:15Z
dc.date.issued2020
dc.departmentFakülteler, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Beyin ve Sinir Cerrahisi Ana Bilim Dalı
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractOBJECTIVE: The members of the matrix metalloproteinase (MMP) family and cannabinoids (CBs) are reportedly associated with hippocampus-dependent memory functions. However, the effects of endogenously formed CBs on hippocampal long-term potentiation remain unknown. The present study aimed to investigate the changes in the gene and protein expression levels of matrix metallopeptidase 9 (MMP-9), phosphatase and tensin homolog (PTEN), and NOTCH receptor 1 (NOTCH1) in rat hippocampal tissues treated with anandamide (AEA), AM251. 6-iodopravadolin (AM630). and N-[4-{[(3,4-Dimethyl-5-isoxazolyl)amino]sulfonyl}phenyl] (ML193). MATERIALS AND METHODS: The subjects were divided into 10 groups (n = five per group). The pharmaceuticals were administered via intraperitoneal injection once a day for seven days, except for the control group. The resected hippocampal tissues were then evaluated using a quantitative real-time polymerase chain reaction (RT-qPCR) and Western blot analysis. The data obtained were statistically analyzed. and p < 0.01 was considered statistically significant. RESULTS: Contrary to the literature, the changes in MMP-9 expression were not statistically significant, but the changes in PTEN and NOTCH1 were. The findings of this in vivo experimental study revealed that the agonists and antagonists acting on the CB system have significant molecular effects on hippocampal tissue. CONCLUSIONS: The changes in gene and protein expressions may be one of the reasons for the neurodegenerative processes observed in patients using these agonists and antagonists, whose effects on the CB system have not been fully explained yet. Our study can contribute to the literature as it is the first study investigating the MMP-9, PTEN and NOTCH1 gene and protein expression.
dc.description.sponsorshipMedical Research Center laboratory (Istanbul Medipol University)
dc.description.sponsorshipThe authors thank the Medical Research Center laboratory (Istanbul Medipol University) employees for all support and assistance
dc.identifier.doi10.26355/eurrev_202011_23845
dc.identifier.endpage11882
dc.identifier.issn1128-3602
dc.identifier.issue22en_US
dc.identifier.pmid33275258
dc.identifier.scopus2-s2.0-85097147802
dc.identifier.scopusqualityQ2
dc.identifier.startpage11871
dc.identifier.urihttps://doi.org/10.26355/eurrev_202011_23845
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5468
dc.identifier.volume24
dc.identifier.wosWOS:000594462100060
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKaraarslan, Numan
dc.institutionauthorŞirin, Duygu Yaşar
dc.language.isoen
dc.publisherVerduci Publisher
dc.relation.ispartofEuropean Review For Medical and Pharmacological Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAnandamide
dc.subjectHippocampus
dc.subjectMMP-9
dc.subjectNOTCH1
dc.subjectPTEN
dc.subjectLong-Term Potentiation
dc.subjectEndocannabinoid System
dc.subjectCannabinoid Receptors
dc.subjectEpilepsy
dc.subjectAgonist
dc.subjectMemory
dc.subjectCb1
dc.subjectInhibition
dc.subjectActivation
dc.subjectMechanisms
dc.titlePharmaco-molecular assessment of the effects of anandamide and its antagonists on hippocampal tissue in Wistar albino rats
dc.typeArticle

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