Use of Nssr Markers for Determination of Clonal Identity and Genetic Structure in A Pinus Brutia Ten. Clonal Seed Orchard

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dc.authorid0000-0002-3227-6680
dc.authorid0000-0001-8323-2509
dc.authorwosidKaya, Nuray/C-1834-2016
dc.authorwosidbilgen, behiye banu/AAD-9188-2019
dc.contributor.authorBilgen, Behiye Banu
dc.contributor.authorKaya, Nuray
dc.date.accessioned2022-05-11T14:45:25Z
dc.date.available2022-05-11T14:45:25Z
dc.date.issued2016
dc.departmentFakülteler, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü
dc.description.abstractDetermination of clonal identity of seed orchard clones is required for the forest tree breeding programs, mating system and pollen contamination studies. Clonal identity and genetic structure in a Pinus brutia Ten. clonal seed orchard were analyzed by 5 nSSR loci (ITPH4516, FRPP94, PEST2669, NZPR544 and EPI3). Needles from 5 ramets per clone (totally 30 clones) were sampled from the seed orchard for microsatellite analysis. Considering the studied ramets of the P. brutia seed orchard clones, all the studied nSSR loci revealed polymorphism. nSSR analysis clearly indicate that the possible multigenotype number of the seed orchard clones (30) was 13 with 7 of these being unique. 39 out of 150 ramets (26%) did not completely match with the genotypes of their supposedly related clones. In 9 out of the 30 clones, all 5 ramets matched each other and in 10 out of the 30 clones, 1 of the 5 ramets did not match the other 4 ramets. This indicates these ramets were mis-lanted or mislabeled during and/or before planting in the orchard. We conclude that genetic identification of orchard ramets seems to be necessary before beginning mating system, pollen contamination studies and forest tree breeding programs.
dc.description.sponsorshipAkdeniz University, Scientific Research Projects UnitAkdeniz University [2008.03.0121.005]
dc.description.sponsorshipThis study was supported by the Akdeniz University, Scientific Research Projects Unit (Project No.: 2008.03.0121.005). Anonymous reviewers provided valuable comments to improve the manuscript. We gratefully acknowledge the contributions of all these persons and institutions.
dc.identifier.endpage3693
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue9en_US
dc.identifier.scopus2-s2.0-84997120326
dc.identifier.scopusqualityN/A
dc.identifier.startpage3687
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10039
dc.identifier.volume25
dc.identifier.wosWOS:000384966000047
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorBilgen, Behiye Banu
dc.language.isoen
dc.publisherParlar Scientific Publications (P S P)
dc.relation.ispartofFresenius Environmental Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectgenetic identity
dc.subjectmislabeling
dc.subjectmultilocus genotype
dc.subjectnuclear microsatellite markers
dc.subjectPinus brutia
dc.subjectPollen Contamination
dc.subjectChloroplast Microsatellites
dc.subjectFertility Variation
dc.subjectRapd Markers
dc.subjectDouglas-Fir
dc.subjectPicea-Abies
dc.subjectSsr Markers
dc.subjectProgeny
dc.subjectSylvestris
dc.subjectManagement
dc.titleUse of Nssr Markers for Determination of Clonal Identity and Genetic Structure in A Pinus Brutia Ten. Clonal Seed Orchard
dc.typeArticle

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