Karayemiş (Prunus laurocerasus L.) bitkisinin ın vıtro doku kültürü optimizasyonu
Küçük Resim Yok
Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Tekirdağ Namık Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Karayemiş (Prunus laurocerasus L.), peyzaj düzenlemesinde parlak, koyu yeşil yaprakları ve kokulu beyaz çiçekleri ile süs değeri açısından ünlüdür. Ancak, estetik çekiciliğinin ötesinde, karayemiş, biyolojik önemine katkıda bulunan çeşitli fitokimyasal özelliklere sahiptir. Başlıca fitokimyasallarından biri, amygdalin gibi siyanojenik glikozitlerdir. Ayrıca çeşitli farmakolojik aktiviteler sunan triterpenoidler, flavonoidler, fenolik bileşikler ve tanenler içerir. Karayemişin çoğaltımı, doğal olarak tohum dağılımı yoluyla veya yapay olarak vejetatif çoğaltma yöntemleriyle gerçekleşebilir. Tohum dağılımı, uygun habitatlarda genetik çeşitliliği ve doğal çoğalımı sağlar. Aynı zamanda, in vitro mikroçoğaltma, karayemişin çoğaltılması, korunması ve genetik iyileştirilmesi için önemli avantajlar sunar, sürdürülebilir yetiştirme uygulamalarını ve çeşitli pazar taleplerini karşılamak üzere yeni çeşitlerin geliştirilmesini kolaylaştırır. Bu çalışmada, Kırklareli ilinin Lüleburgaz ilçesinden toplanan Karayemiş (Prunus laurocerasus L.) bitkilerinin sürgün uçlarını kullanarak farklı bitki büyüme düzenleyicileri kombinasyonları ile bitkinin in vitro mikroçoğaltımı amaçlanmıştır. Dış ortam koşullarından toplanan eksplantların sterilizasyon aşamasında civa klorür (HgCl2) kullanılmış ve %0,1 HgCl2 içeren ortamdan 8 dakika sonra başarılı sonuç elde edilmiştir. En iyi çoğaltım sonucu, 1 mg/l BAP, 0,5 mg/l GA3 ve 0,1 mg/l IBA içeren MS ortamından elde edilmiştir. 21 gün sonra 5 cm uzunluğa ulaşan sürgünler köklendirme ortamına aktarılmıştır. Tüm ortamlarda dört hafta içinde köklenme gözlenmiş olup, en güçlü ve uzun kök, 2 mg/l IBA, 0,5 mg/l GA3 ve 0,15 g/l Fe-EDDHA içeren MS 0 ortamından elde edilmiştir. Son olarak, köklenen bitkiler seradaki saksılara aktarılmıştır. Anahtar Kelimeler: Karayemiş, in vitro, Mikroçoğaltım, Köklendirme, Süs Bitkisi, Fe-EDDHA
Cherry laurel (Prunus laurocerasus L.) is renowned for its ornamental value in landscapingdue to its glossy, dark green leaves and fragrant white flowers. However, beyond its aesthetic appeal, cherry laurel possesses several phytochemical properties contributing to its biological significance. One of the primary phytochemicals in cherry laurel is cyanogenic glycosides, such as amygdalin. Cherry laurel contains triterpenoids, flavonoids, phenolic compounds, and tannins, which offer various pharmacological activities. Cherry laurel regeneration can ocur naturally through seed dispersal or artificially through vegetative propagation methods. Seed dispersal facilitates genetic diversity and natural regeneration in suitable habitats. At the same time, in vitro micropropagation offers significant advantages for the propagation, conservation, and genetic improvement of cherry laurel, facilitating sustainable cultivation practices and the development of novel varieties to meet diverse market demands. The aim of the study was to conduct an in vitro micropropagation experiment using shoot tips of Cherry laurel (Prunus laurocerasus L.) plants collected from Lüleburgaz district of Kırklareli province, Turkey, with different combinations of plant growth regulators. During the sterilization stage of the collected explants from outdoor conditions, mercury chloride (HgCl2) was utilized, and the successful result was obtained from a medium containing 0,1% HgCl2for 8 minutes. The best regeneration result was achieved from MS medium containing 1 mg/l BAP, 0.5 mg/l GA3, and 0.1 mg/l IBA. Shoots reaching a length of 5 cm after 21 days were transferred to a rooting medium. Rooting was observed in all mediums within four weeks, with The strongest and longest root was obtained from 0 MS medium containing 2 mg/l IBA, 0,5 mg/l GA3, and 0,15 g/l Fe-EDDHA. Finally, rooted plants were transferred to pots in the greenhouse. Keywords: Cheery Laurel, in vitro, Micropropagation, Rooting, Ornamental Plant, Fe-EDDHA
Cherry laurel (Prunus laurocerasus L.) is renowned for its ornamental value in landscapingdue to its glossy, dark green leaves and fragrant white flowers. However, beyond its aesthetic appeal, cherry laurel possesses several phytochemical properties contributing to its biological significance. One of the primary phytochemicals in cherry laurel is cyanogenic glycosides, such as amygdalin. Cherry laurel contains triterpenoids, flavonoids, phenolic compounds, and tannins, which offer various pharmacological activities. Cherry laurel regeneration can ocur naturally through seed dispersal or artificially through vegetative propagation methods. Seed dispersal facilitates genetic diversity and natural regeneration in suitable habitats. At the same time, in vitro micropropagation offers significant advantages for the propagation, conservation, and genetic improvement of cherry laurel, facilitating sustainable cultivation practices and the development of novel varieties to meet diverse market demands. The aim of the study was to conduct an in vitro micropropagation experiment using shoot tips of Cherry laurel (Prunus laurocerasus L.) plants collected from Lüleburgaz district of Kırklareli province, Turkey, with different combinations of plant growth regulators. During the sterilization stage of the collected explants from outdoor conditions, mercury chloride (HgCl2) was utilized, and the successful result was obtained from a medium containing 0,1% HgCl2for 8 minutes. The best regeneration result was achieved from MS medium containing 1 mg/l BAP, 0.5 mg/l GA3, and 0.1 mg/l IBA. Shoots reaching a length of 5 cm after 21 days were transferred to a rooting medium. Rooting was observed in all mediums within four weeks, with The strongest and longest root was obtained from 0 MS medium containing 2 mg/l IBA, 0,5 mg/l GA3, and 0,15 g/l Fe-EDDHA. Finally, rooted plants were transferred to pots in the greenhouse. Keywords: Cheery Laurel, in vitro, Micropropagation, Rooting, Ornamental Plant, Fe-EDDHA
Açıklama
Fen Bilimleri Enstitüsü, Tarımsal Biyoteknoloji Ana Bilim Dalı
Anahtar Kelimeler
Biyoteknoloji, Biotechnology ; Ziraat
