Identification and Characterization of Carnitine Palmitoyltransferase 1A (CPT1A) Gene in European Sea Bass (Dicentrarchus labrax)
Küçük Resim Yok
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: CPT1A intervenes in the access of the long-chain fatty acyl-CoA to the mitochondrial matrix. In this study, we conducted the genetic polymorphisms of the partial sequence of the CPT1A gene in European sea bass (Dicentrarchus labrax) that were cage cultured in the Aegean Sea. Material and Methods: Genomic DNA was isolated from 100 European sea bass individuals. After amplifying a partial region of the CPT1A gene by thermal cycler, PCR products were sequenced via Sanger method. Results: We detected two SNPs in partial sequence of the CPT1A gene in European sea bass. TT, TA and AA genotypes were observed for CPT1A g.2080T>A locus with frequencies of 27.04, 49.92 and 23.04%, respectively. The frequencies of the g.2216A>G locus as AA, AG and GG were found to be 1.2, 19.58 and 79.21%, respectively. The CPT1A g.2080T>A locus was in HWE, whereas the g.2216A>G locus was not in HWE. Conclusion: European sea bass has a high commercial value. These findings suggest that two SNPs in the CPT1A gene could be used for genomic selection programs related to fatty acid composition in European sea bass. Keywords: Fatty acid, SNP, sea bass, variation
Açıklama
Anahtar Kelimeler
SNP, fatty acid, variation, sea bass
Kaynak
Hayvansal Üretim
WoS Q Değeri
Scopus Q Değeri
Cilt
64
Sayı
1
