A Simple and Low-Cost Electrochemical Immunosensor for Ultrasensitive Determination of Calreticulin Biomarker in Human Serum

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dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2023-05-06T17:23:45Z
dc.date.available2023-05-06T17:23:45Z
dc.date.issued2023
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractAn early on time detection of breast cancer significantly affects the treatment process and outcome. Herein, a new label-free impedimetric biosensor is developed to determine the lowest change in the level of calreticulin (CALR), which is a new biomarker of breast carcinoma. The proposed immunosensor is fabricated by using reduced graphene oxide/amino substituted polypyrrole polymer (rGO-PPyNH2) nanocomposite modified disposable electrode. The anti-CALR antibodies are first attached on the rGO-PPyNH2 nanocomposite coated electrode through glutaraldehyde crosslinking; the CALR antigens are then immobilized with the addition of CALR antigens to form an immunocomplex on the sensing surface. This immunocomplex induces considerably larger interfacial electron transport resistance (R-ct). The variation in the R-ct has a linear relationship with CALR level in the detection range of 0.025 to 75 pg mL(-1), with a detection limit of 10.4 fg mL(-1). The suggested biosensor shows high selectivity to CALR, good storage stability (at least 5 weeks) and suitable reproducibility results as shown in quality control chart. The designed immunosensor is utilized to analyze CALR levels in human sera with satisfying results. This immunosensor provides a novel way for the clinical determination of CALR and other cancer biological markers.
dc.description.sponsorshipScientific and Technological Research Council of Tekirdag Namik Kemal University, Turkey; [NKUBAP.00]
dc.description.sponsorshipAcknowledgements This work was supported by the Scientific and Technological Research Council of Tekirdag Namik Kemal University, Turkey (Project number NKUBAP.00.GA.21.328).
dc.identifier.doi10.1002/mabi.202200390
dc.identifier.issn1616-5187
dc.identifier.issn1616-5195
dc.identifier.issue1en_US
dc.identifier.pmid36419333
dc.identifier.scopus2-s2.0-85143974323
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1002/mabi.202200390
dc.identifier.urihttps://hdl.handle.net/20.500.11776/12288
dc.identifier.volume23
dc.identifier.wosWOS:000893339200001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Elif Burcu
dc.institutionauthorAydın, Muhammet
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofMacromolecular Bioscience
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectcalreticulin
dc.subjectimpedimetric immunosensor
dc.subjectreagentless analysis
dc.subjectreduced graphene oxide
dc.subjectGraphene Oxide
dc.subjectFunctionalized Graphene
dc.subjectRaman-Spectroscopy
dc.subjectLabel-Free
dc.subjectBiosensor
dc.subjectPolymer
dc.subjectImmobilization
dc.subjectSurface
dc.subjectCancer
dc.subjectReduction
dc.titleA Simple and Low-Cost Electrochemical Immunosensor for Ultrasensitive Determination of Calreticulin Biomarker in Human Serum
dc.typeArticle

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