A Simple and Low-Cost Electrochemical Immunosensor for Ultrasensitive Determination of Calreticulin Biomarker in Human Serum
Yükleniyor...
Dosyalar
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
An early on time detection of breast cancer significantly affects the treatment process and outcome. Herein, a new label-free impedimetric biosensor is developed to determine the lowest change in the level of calreticulin (CALR), which is a new biomarker of breast carcinoma. The proposed immunosensor is fabricated by using reduced graphene oxide/amino substituted polypyrrole polymer (rGO-PPyNH2) nanocomposite modified disposable electrode. The anti-CALR antibodies are first attached on the rGO-PPyNH2 nanocomposite coated electrode through glutaraldehyde crosslinking; the CALR antigens are then immobilized with the addition of CALR antigens to form an immunocomplex on the sensing surface. This immunocomplex induces considerably larger interfacial electron transport resistance (R-ct). The variation in the R-ct has a linear relationship with CALR level in the detection range of 0.025 to 75 pg mL(-1), with a detection limit of 10.4 fg mL(-1). The suggested biosensor shows high selectivity to CALR, good storage stability (at least 5 weeks) and suitable reproducibility results as shown in quality control chart. The designed immunosensor is utilized to analyze CALR levels in human sera with satisfying results. This immunosensor provides a novel way for the clinical determination of CALR and other cancer biological markers.
Açıklama
Anahtar Kelimeler
calreticulin, impedimetric immunosensor, reagentless analysis, reduced graphene oxide, Graphene Oxide, Functionalized Graphene, Raman-Spectroscopy, Label-Free, Biosensor, Polymer, Immobilization, Surface, Cancer, Reduction
Kaynak
Macromolecular Bioscience
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
23
Sayı
1
