Polymorphism of sheep POU1F1 gene exon 6 and 3'UTR region and their association with milk production traits

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dc.authorid0000-0001-7460-4040
dc.authorid0000-0002-8577-7323
dc.authorwosidÜnal, Emel Özkan/ABA-3367-2020
dc.authorwosidKul, Selim/W-3852-2018
dc.contributor.authorÖzmen, O.
dc.contributor.authorKul, Seval
dc.contributor.authorÖzkan Ünal, Emel
dc.date.accessioned2022-05-11T14:46:32Z
dc.date.available2022-05-11T14:46:32Z
dc.date.issued2014
dc.departmentFakülteler, Ziraat Fakültesi, Zootekni Bölümü
dc.description.abstractThe purpose of the study was to detect the AluI and DdeI polymorphisms within POU1F1 gene exon 6 and 3'UTR region in Turkish sheep breeds, and their association with milk productive traits. Jugular blood samples were collected from 50 Sakiz, 50 White Karaman, and 50 Awassi sheep using EDTA as an anticoagulant. PCR-RFLP and sequencing analysis were performed to investigate possible polymorphisms in the exon 6 and 3' flanking region of the sheep POU1F1 gene. The PCR products were digested with restriction endonuclease AluI and DdeI, and biallelic polymorphism was found with restriction endonuclease AluI and two genotypes (TT (296 bp and 173 bp) and CC (235 bp, 173 bp and 61 bp)) were detected. White Karaman and Awassi breeds did not show polymorphisms for AluI restriction sites. No polymorphism at the DdeI cleavage sites was detected in the three sheep breeds. Significant statistical results were found in milk yield (***P<0.001), fat (***P<0.001) and lactose (*P<0.05) values with TT and CC genotypes, however no significant association of TT and CC genotypes with protein values was detected (P>0.05) and individuals with genotype TT had a superior milk yield in Sakiz sheep breeds. As sequence results, seven variation points were determined for exon 6 (g.185T>C) and 3'UTR (g.220G>A, g.229C>T, g.248C>T, g.250A>T, g.255T>C, g.258C>T) of the sheep POU1F1 gene. We have reported here for the first time single nucleotide polymorphisms of the POU1F1 gene for both exon 6 and 3'UTR and its effects on milk traits in Turkish sheep breeds were evaluated.
dc.description.sponsorshipScientific Research Projects Council of Firat UniversityFirat University [2110]
dc.description.sponsorshipThe project was supported by Scientific Research Projects Council of Firat University (project code 2110).
dc.identifier.endpage335
dc.identifier.issn1728-1997
dc.identifier.issue4en_US
dc.identifier.pmid27175126
dc.identifier.scopus2-s2.0-84922912320
dc.identifier.scopusqualityQ2
dc.identifier.startpage331
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10318
dc.identifier.volume15
dc.identifier.wosWOS:000348616300003
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzkan Ünal, Emel
dc.language.isoen
dc.publisherShiraz Univ
dc.relation.ispartofIranian Journal of Veterinary Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectMilk traits
dc.subjectPOU1F1 gene
dc.subjectPolymorphism
dc.subjectTurkish sheep breeds
dc.subjectLocus
dc.titlePolymorphism of sheep POU1F1 gene exon 6 and 3'UTR region and their association with milk production traits
dc.typeArticle

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