Quantitative comparison of different purification and detection methods for Cryptosporidium parvum oocysts

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dc.authorid0000-0003-3856-1850
dc.authorscopusid15769247000
dc.authorscopusid24831873600
dc.authorscopusid56218530300
dc.authorscopusid8941775200
dc.contributor.authorKar, Sırrı
dc.contributor.authorGawlowska, Sandra
dc.contributor.authorDaugschies, Arwid
dc.contributor.authorBangoura, Befit
dc.date.accessioned2022-05-11T14:28:27Z
dc.date.available2022-05-11T14:28:27Z
dc.date.issued2011
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractCryptosporidium parvum (C. parvum) is the causal agent of cryptosporidiosis in many animals, mainly cattle, and possesses a high zoonotic potential. It occurs worldwide and ubiquitously. Detection of C. parvum is mainly performed directly but purification of the oocysts is useful to increase sensitivity and to obtain oocyst material for further use. The study was designed to compare (a) three different direct diagnostic methods, namely modified Ziehl-Neelsen staining, carbol fuchsin staining and conventional PCR, and (b) three routine oocyst purification methods, in particular flotation with saturated sodium chloride solution, Sheather's sucrose solution and a Percoll gradient. During comparison of purification methods, special regard was paid to the ability to separate morphologically intact oocysts from the morphologically degenerated fraction or viable from non-viable oocysts, respectively. Results: (a) Diagnostic methods: Most effective in C. parvum oocysts detection in calf faeces was PCR; carbol fuchsin and modified Ziehl-Neelsen stainings achieved comparable results. (b) Purification methods: Oocyst flotation using sodium chloride solution showed to be superior to Percoll gradient centrifugation and sugar flotation in terms of purification quality, recovery efficacy (yield) and reduction of the proportion of degenerated or non-viable oocysts. (C) 2010 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.vetpar.2010.12.005
dc.identifier.endpage370
dc.identifier.issn0304-4017
dc.identifier.issue45385en_US
dc.identifier.pmid21242035
dc.identifier.scopus2-s2.0-79954605211
dc.identifier.scopusqualityQ1
dc.identifier.startpage366
dc.identifier.urihttps://doi.org/10.1016/j.vetpar.2010.12.005
dc.identifier.urihttps://hdl.handle.net/20.500.11776/6836
dc.identifier.volume177
dc.identifier.wosWOS:000290696800023
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKar, Sırrı
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofVeterinary Parasitology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCryptosporidium parvum
dc.subjectCarbol fuchsin
dc.subjectModified Ziehl-Neelsen staining
dc.subjectPCR
dc.subjectFlotation methods
dc.subjectEfficiency
dc.subjectFeces
dc.subjectViability
dc.titleQuantitative comparison of different purification and detection methods for Cryptosporidium parvum oocysts
dc.typeArticle

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