Quantitative Analysis of a Promising Cancer Biomarker, Calretinin, by a Biosensing System Based on Simple and Effective Immobilization Process

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dc.authorid0000-0002-6232-3388
dc.authorscopusid25935968900
dc.authorscopusid6603077608
dc.authorscopusid6506764918
dc.authorwosidAsav, Engin/AAG-1170-2021
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.authorwosidAsav, Engin/AAG-4182-2021
dc.contributor.authorAsav, Engin
dc.contributor.authorSağıroğlu, Ayten
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:30:55Z
dc.date.available2022-05-11T14:30:55Z
dc.date.issued2016
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractCalretinin (CAL) is calcium binding protein, and its levels in blood and cerebrospinal fluids are increased, since its expression is increased various cancer types. A novel biosensor system fabricated by immobilization of a specific antibody to CAL, anti-Calretinin (anti-CAL), onto a gold electrode surface via an effective covalent binding method using mercaptohexanol, epichlorohydrin, and ethanolamine was reported for the sensitive, selective, and accurate analysis of CAL. The proposed biosensor showed a linear calibration range between 1 ng/mL and 5 ng/mL. LOD and LOQ values were determined as 0.11 ng/mL and 0.38 ng/mL, respectively. The standard deviation related to the reproducibility of the new biosensor system was calculated as 3.95%. Lastly, in order to state the applicability of the biosensor to early diagnosis of CAL in practice, artificial serum samples spiked with CAL have been analyzed by the proposed biosensor.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113Z 678]
dc.description.sponsorshipThis work was funded by TUBITAK (The Scientific and Technological Research Council of Turkey, Project numbers: 113Z 678).
dc.identifier.doi10.1002/elan.201500324
dc.identifier.endpage342
dc.identifier.issn1040-0397
dc.identifier.issn1521-4109
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84963657222
dc.identifier.scopusqualityQ2
dc.identifier.startpage334
dc.identifier.urihttps://doi.org/10.1002/elan.201500324
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7234
dc.identifier.volume28
dc.identifier.wosWOS:000370138000011
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofElectroanalysis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCalretinin
dc.subjectepichlorohydrin
dc.subjectcancer biomarker
dc.subjectimmunosensor
dc.subjectElectrochemical Impedance Spectroscopy
dc.subjectSelf-Assembled Monolayers
dc.subjectSurface-Plasmon Resonance
dc.subjectHydroxyl-Groups
dc.subjectProtein
dc.subjectGold
dc.subjectExpression
dc.subjectActivation
dc.subjectImmunohistochemistry
dc.subjectAdsorption
dc.subjectDiagnosis
dc.titleQuantitative Analysis of a Promising Cancer Biomarker, Calretinin, by a Biosensing System Based on Simple and Effective Immobilization Process
dc.typeArticle

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