EFFECT OF PARAMETERS ON REMOVAL OF RB19 DYE BY LACCASE ENZYME AND KINETIC EVALUATION

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dc.authorid0000-0002-1457-1504
dc.authorwosidCifci, Deniz Izlen/AAH-3383-2020
dc.authorwosidGüneş, Elçin/ABA-1276-2020
dc.authorwosidGüneş, Elçin/ABA-2259-2020
dc.authorwosidGunes, Yalcin/ABA-1290-2020
dc.contributor.authorÇifçi, Deniz İzlen
dc.contributor.authorAtav, Reza
dc.contributor.authorGüneş, Yalçın
dc.contributor.authorGüneş, Elçin
dc.date.accessioned2022-05-11T14:02:46Z
dc.date.available2022-05-11T14:02:46Z
dc.date.issued2020
dc.departmentFakülteler, Çorlu Mühendislik Fakültesi, Tekstil Mühendisliği Bölümü
dc.departmentFakülteler, Çorlu Mühendislik Fakültesi, Çevre Mühendisliği Bölümü
dc.description.abstractDye decolorization of RB19 using commercial laccase (Cerrena unicolor) enzyme was evaluated in this study. The effect of pH, laccase concentration, temperature, metal salt, carbon and nitrogen sources and solvents on the removal of RB19 dye were examined in detail. Highest RB19 removal with laccase (Cerrena unicolor) was obtained at pH 6, 40 degrees C, 0.3 g/L enzyme concentration and 200 mg/L initial dye concentration. According to the Michaelis-Menten kinetics, the highest V-max was 6.61 mg/L.min at these conditions. 0.25 mM of Cu2+ or Mn2+ enhanced the decolorization of RB19 but further increase in metal concentration decreased the removal of RB19. In the presence of carbon source such as glucose, starch and lactose, removal of RB19 by laccase was not significantly affected. However, in the presence of nitrogen sources such as urea and NH4Cl, the removal of RB19 by laccase was significantly reduced.
dc.identifier.endpage382
dc.identifier.issn1304-7205
dc.identifier.issn1304-7191
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85149902674
dc.identifier.scopusqualityN/A
dc.identifier.startpage371
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4473
dc.identifier.volume38
dc.identifier.wosWOS:000522761700027
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorÇifçi, Deniz İzlen
dc.institutionauthorAtav, Reza
dc.institutionauthorGüneş, Yalçın
dc.institutionauthorGüneş, Elçin
dc.language.isoen
dc.publisherYildiz Technical Univ
dc.relation.ispartofSigma Journal Of Engineering And Natural Sciences-Sigma Muhendislik Ve Fen Bilimleri Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCerrena unicolor
dc.subjectenzymatic treatment
dc.subjectkinetic
dc.subjectlaccase
dc.subjectReactive Blue 19
dc.subjectBrilliant-Blue-R
dc.subjectPleurotus-Ostreatus
dc.subjectSynthetic Dyes
dc.subjectCrude Laccase
dc.subjectTextile Dyes
dc.subjectExtracellular Laccase
dc.subjectRedox Mediators
dc.subjectMetal-Ions
dc.subjectDecolorization
dc.subjectDetoxification
dc.titleEFFECT OF PARAMETERS ON REMOVAL OF RB19 DYE BY LACCASE ENZYME AND KINETIC EVALUATION
dc.typeArticle

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