EFFECT OF PARAMETERS ON REMOVAL OF RB19 DYE BY LACCASE ENZYME AND KINETIC EVALUATION

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Küçük Resim

Tarih

2020

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Yildiz Technical Univ

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Dye decolorization of RB19 using commercial laccase (Cerrena unicolor) enzyme was evaluated in this study. The effect of pH, laccase concentration, temperature, metal salt, carbon and nitrogen sources and solvents on the removal of RB19 dye were examined in detail. Highest RB19 removal with laccase (Cerrena unicolor) was obtained at pH 6, 40 degrees C, 0.3 g/L enzyme concentration and 200 mg/L initial dye concentration. According to the Michaelis-Menten kinetics, the highest V-max was 6.61 mg/L.min at these conditions. 0.25 mM of Cu2+ or Mn2+ enhanced the decolorization of RB19 but further increase in metal concentration decreased the removal of RB19. In the presence of carbon source such as glucose, starch and lactose, removal of RB19 by laccase was not significantly affected. However, in the presence of nitrogen sources such as urea and NH4Cl, the removal of RB19 by laccase was significantly reduced.

Açıklama

Anahtar Kelimeler

Cerrena unicolor, enzymatic treatment, kinetic, laccase, Reactive Blue 19, Brilliant-Blue-R, Pleurotus-Ostreatus, Synthetic Dyes, Crude Laccase, Textile Dyes, Extracellular Laccase, Redox Mediators, Metal-Ions, Decolorization, Detoxification

Kaynak

Sigma Journal Of Engineering And Natural Sciences-Sigma Muhendislik Ve Fen Bilimleri Dergisi

WoS Q Değeri

N/A

Scopus Q Değeri

N/A

Cilt

38

Sayı

1

Künye