Tissue cryobanking for conservation programs: effect of tissue type and storage time after death

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dc.authorid0000-0002-2789-0635
dc.authorid0000-0003-2991-6322
dc.authorscopusid53463162500
dc.authorscopusid23767133200
dc.authorscopusid16400954400
dc.authorscopusid6603553213
dc.authorwosidarat, sezen/ABA-7370-2020
dc.contributor.authorCaputcu, Arzu Tas
dc.contributor.authorAkkoç, Tolga
dc.contributor.authorÇetinkaya, Gaye
dc.contributor.authorArat, Sezen
dc.date.accessioned2022-05-11T14:45:22Z
dc.date.available2022-05-11T14:45:22Z
dc.date.issued2013
dc.departmentFakülteler, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü
dc.description.abstractIn this study, we investigated the temporal post-mortem limits, within which there will be guarantees of obtaining living cells from several tissues of sheep and cattle and the effect of vitrification on the ability of cells from tissue stored at different times. Muscle tissue and auricular cartilage were stored at 4 degrees C for 5, 48, 72, 96 and 216 h post-mortem (hpm). Tissue samples were sorted into two groups: one group was in vitro cultured immediately after storage and the other was vitrified after storage and then in vitro cultured. In cattle and sheep, no differences in subconfluence rates were observed between the two experimental groups. At the same time, no significant differences were observed in the number of days required in culture to reach confluence between non-vitrified and vitrified groups when tissues were stored at 4 degrees C for different times. In sheep, while the population doubling times (PDT) were similar in cartilage cells from vitrified and non-vitrified tissues and stored at 4 degrees C for 5 and 216 hpm, PDT of muscle cells were longer in 216 hpmstored groups than in 5 hpm stored groups. In bovine, although the PDT of muscle cells were similar for 5 and 216 hpm and both vitrified and non-vitrified tissues and the PDT were longer in cartilage cells from vitrified than from non-vitrified tissues. In conclusion, although storage times and vitrification have different effects on tissues from cattle and sheep, this study showed that living cells could be obtained from all groups. Therefore, cartilage andmuscle tissues can be stored at 4 degrees C for 216 hpm and used for cyrobanking.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [KAMAG-106G005]
dc.description.sponsorshipWe would like to thank Dr. Digdem Aktoprakligil Aksu for manuscript review; Fatih Karakaya, Erman Ates and Ozlem Celasin for technical assistance. The manuscript has been edited by native speaker Dr. Anita L. Akkas, who has PhD degree in English Literature and MA degree in Linguistics Engineering and Science. This research was funded by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project no. KAMAG-106G005).
dc.identifier.doi10.1007/s10561-012-9292-6
dc.identifier.endpage10
dc.identifier.issn1389-9333
dc.identifier.issn1573-6814
dc.identifier.issue1en_US
dc.identifier.pmid22271151
dc.identifier.scopus2-s2.0-84873696895
dc.identifier.scopusqualityQ2
dc.identifier.startpage1
dc.identifier.urihttps://doi.org/10.1007/s10561-012-9292-6
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10025
dc.identifier.volume14
dc.identifier.wosWOS:000319424900001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorArat, Sezen
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofCell and Tissue Banking
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCryopreservation
dc.subjectVitrification
dc.subjectCartilage tissue
dc.subjectMuscle tissue
dc.subjectCryobanking
dc.subjectNuclear Transfer Embryos
dc.subjectCumulus Cells
dc.subjectSkin Samples
dc.subjectVitrification
dc.subjectCartilage
dc.subjectCryopreservation
dc.subjectRabbit
dc.subjectAdult
dc.subjectFibroblasts
dc.subjectCloning
dc.titleTissue cryobanking for conservation programs: effect of tissue type and storage time after death
dc.typeArticle

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