In Silico Analysis of the Structural and Functional Consequences of Polymorphic Amino Acid Substitutions in the Cattle HSF1 Protein

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dc.authorscopusid55567956000
dc.contributor.authorAtalay, Sertaç
dc.date.accessioned2023-04-20T08:02:25Z
dc.date.available2023-04-20T08:02:25Z
dc.date.issued2022
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractHeat stress causes a decrease in the productivity of livestock by negatively affecting some important economic features such as fertility, growth and milk production. The heat shock transcription factor 1 (HSF1) gene plays a key role in the regulation of the stress response. Therefore, the present study aimed to predict the most deleterious non-synonymous single nucleotide polymorphisms (nsSNP) on the cattle HSF1 gene via in silico analyses. Out of 170 nsSNPs in the HSF1 gene, 14 SNPs were predicted as deleterious by all the nine the vast majority of SNPs predicted to be deleterious were evolutionary conserved. Protein structural analyses were performed I-Mutant, Mupro, Hope Project server, RaptorX and Swiss Model server. The 12 amino acid substitutions (V15G, F18L, L19R, K21M, I35T, V46E, V56G, F61L, A67D, Y76D, V81G, L112P) in the DNA binding region of the cattle HSF1 protein were predicted to be highly deleterious. The P112 variant was predicted to disrupt an a-helix structure. It was determined that the two amino acid changes (K21M, Y76D) on the surface of the protein were different in terms of hydrophobicity, charge, and size. These variants (M21, D76) might hamper the protein's interaction with the heat shock elements.
dc.identifier.doi10.9775/kvfd.2022.27152
dc.identifier.issn1300-6045
dc.identifier.scopus2-s2.0-85134589435
dc.identifier.scopusqualityQ3
dc.identifier.trdizinid532235
dc.identifier.urihttps://doi.org/10.9775/kvfd.2022.27152
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10904
dc.identifier.wosWOS:000800606100001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorAtalay, Sertaç
dc.language.isoen
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisi
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHsfi
dc.subjectHeat Stress
dc.subjectCattle
dc.subjectNssnp
dc.subjectHsp
dc.subjectStability Changes
dc.subjectAssociation
dc.subjectPrediction
dc.subjectMutations
dc.subjectVariants
dc.subjectSequence
dc.subjectDisease
dc.titleIn Silico Analysis of the Structural and Functional Consequences of Polymorphic Amino Acid Substitutions in the Cattle HSF1 Protein
dc.typeArticle

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