Investigation of the Effects of Methylphenidate, an Amphetamine Derivative, on Intervertebral Disc Tissue Cell Cultures and Matrix Structures

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dc.authorid0000-0002-8084-7855
dc.authorid0000-0003-2003-6337
dc.authorscopusid57194611714
dc.authorscopusid56674583800
dc.authorscopusid56769801000
dc.authorscopusid7005912992
dc.authorscopusid57201362754
dc.authorscopusid35197435300
dc.authorwosidOzbek, Hanefi/O-3472-2019
dc.authorwosidsirin, duygu yasar/AAR-8685-2020
dc.contributor.authorKaya, Yasin Emre
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorYasar Şirin, Duygu
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorKaplan, Necati
dc.contributor.authorYılmaz, İbrahim
dc.date.accessioned2022-05-11T14:34:48Z
dc.date.available2022-05-11T14:34:48Z
dc.date.issued2019
dc.departmentFakülteler, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Beyin ve Sinir Cerrahisi Ana Bilim Dalı
dc.description.abstractAIM: To investigate the effects of methylphenidate (MPH), on intervertebral disc tissue (IVD) cell cultures and extracellular matrix structures. Changes in the expression of some important marker genes involved in anabolic and catabolic mechanisms of IVD extracellular matrix formation were also evaluated. MATERIAL and METHODS: Primary cultures of nucleus pulposus cells (NPCs) and annulus fibrosus cells (AFCs) were isolated from tissues obtained from the operated patients. Cell viability and proliferation were tested, and the cell surface morphologies were evaluated by microscopy. The expressions of the chondroadherin (CHAD), cartilage oligomeric matrix protein (COMP), interleukin-1 beta (IL-1 beta) and matrix metalloproteinase (M MP) -7 and M MP-19 genes were evaluated using the quantitative real-time polymerase chain reaction (qRT-PCR). A value of p<0.05 was considered statistically significant. RESULTS: The viability and proliferation of intervertebral disc tissue cells decreased in response to MPH treatment and the expression of the investigated genes also changed. CONCLUSION: The data obtained from in-vitro studies may not directly adaptable to clinical applications. However, the fact that the central nervous system stimulant MPH can suppress proliferation of cells derived from IVD tissue should be considered carefully by clinicians.
dc.identifier.doi10.5137/1019-5149.JTN.26339-19.2
dc.identifier.endpage742
dc.identifier.issn1019-5149
dc.identifier.issue5en_US
dc.identifier.pmid31353439
dc.identifier.scopus2-s2.0-85072350718
dc.identifier.scopusqualityQ3
dc.identifier.startpage734
dc.identifier.urihttps://doi.org/10.5137/1019-5149.JTN.26339-19.2
dc.identifier.urihttps://hdl.handle.net/20.500.11776/8096
dc.identifier.volume29
dc.identifier.wosWOS:000486326100017
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKaraarslan, Numan
dc.language.isoen
dc.publisherTurkish Neurosurgical Soc
dc.relation.ispartofTurkish Neurosurgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCartilage oligomeric matrix protein
dc.subjectChondroadherin
dc.subjectInterleukin-1 beta
dc.subjectIntervertebral disc
dc.subjectMatrix metalloproteinase
dc.subjectMethylphenidate
dc.subjectDisorder
dc.subjectAdhd
dc.titleInvestigation of the Effects of Methylphenidate, an Amphetamine Derivative, on Intervertebral Disc Tissue Cell Cultures and Matrix Structures
dc.typeArticle

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