A label-free immunosensor for sensitive detection of RACK 1 cancer biomarker based on conjugated polymer modified ITO electrode

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dc.authorscopusid57194273220
dc.authorscopusid55571487900
dc.authorscopusid6506764918
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:07:23Z
dc.date.available2022-05-11T14:07:23Z
dc.date.issued2020
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractA new flexible biosensor based on conjugated polymer functionalized indium tin oxide (ITO) sheet was fabricated for Receptor for Activated C Kinase 1 (RACK 1) determination. Poly(3-thiophene acetic acid) (P(Thi-Ac)) was used as an immobilization matrix for construction of RACK 1 immunosensor. This polymer had a great number of carboxyl groups on its end site and these carboxyl ends provided anchoring points to the anti-RACK 1 antibodies. Anti-RACK 1 antibodies were covalently attached on the ITO electrode and recognized the RACK 1 antigens. Electrochemical characterizations were made by employing electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. Additionally, single frequency impedance method (SFI) was applied to follow the specific biointeraction between antibody and antigen. As a result of specific biointeraction, the designed immunosensor exhibited a wide linear detection range between 0.01 pg/mL and 2 pg/mL RACK 1 with a detection limit of 3.1 fg/mL. Scanning electron microscopy and atomic force microscopy analyses were employed for electrode surface morphology investigation. The designed RACK 1 biosensor had good repeatability (5.73 %, RSD), excellent reproducibility (2.5 %, RSD), long storage-stability and reusable property. In addition, the fabricated RACK 1 biosensor was applied to determine RACK 1 concentration in human serums and the recovery was ranging from 98.79%–100.22%. This work illustrated a new tool to construct a sensitive and low-cost disposable biosensor for applications in clinical monitoring. © 2020 Elsevier B.V.
dc.identifier.doi10.1016/j.jpba.2020.113517
dc.identifier.issn0731-7085
dc.identifier.pmid32784093
dc.identifier.scopus2-s2.0-85089193259
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.jpba.2020.113517
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5091
dc.identifier.volume190
dc.identifier.wosWOS:000573434900017
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Elif Burcu
dc.institutionauthorAydın, Muhammet
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCancer biomarker
dc.subjectConjugated polymer
dc.subjectElectrochemical biosensor
dc.subjectElectrochemical impedance spectroscopy
dc.subjectReceptor for activated C kinase 1
dc.subjectimmobilized antibody
dc.subjectindium tin oxide
dc.subjectpoly(3 thiophene acetic acid)
dc.subjectpolyethylene terephthalate
dc.subjectpolymer
dc.subjectprotein antibody
dc.subjectreceptor for activated C kinase 1
dc.subjectunclassified drug
dc.subjectindium tin oxide
dc.subjectpolymer
dc.subjecttin derivative
dc.subjecttumor marker
dc.subjectantigen detection
dc.subjectArticle
dc.subjectatomic force microscopy
dc.subjectconjugation
dc.subjectcontrolled study
dc.subjectcyclic voltammetry
dc.subjectelectrochemical detection
dc.subjecthuman
dc.subjectimmobilization
dc.subjectimpedance spectroscopy
dc.subjectlimit of detection
dc.subjectlimit of quantitation
dc.subjectpriority journal
dc.subjectscanning electron microscopy
dc.subjectserum
dc.subjectelectrochemical analysis
dc.subjectelectrode
dc.subjectgenetic procedures
dc.subjectimmunoassay
dc.subjectneoplasm
dc.subjectreproducibility
dc.subjectAntibodies, Immobilized
dc.subjectBiomarkers, Tumor
dc.subjectBiosensing Techniques
dc.subjectElectrochemical Techniques
dc.subjectElectrodes
dc.subjectHumans
dc.subjectImmunoassay
dc.subjectLimit of Detection
dc.subjectNeoplasms
dc.subjectPolymers
dc.subjectReproducibility of Results
dc.subjectTin Compounds
dc.titleA label-free immunosensor for sensitive detection of RACK 1 cancer biomarker based on conjugated polymer modified ITO electrode
dc.typeArticle

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