A label-free immunosensor for sensitive detection of RACK 1 cancer biomarker based on conjugated polymer modified ITO electrode
Yükleniyor...
Dosyalar
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier B.V.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A new flexible biosensor based on conjugated polymer functionalized indium tin oxide (ITO) sheet was fabricated for Receptor for Activated C Kinase 1 (RACK 1) determination. Poly(3-thiophene acetic acid) (P(Thi-Ac)) was used as an immobilization matrix for construction of RACK 1 immunosensor. This polymer had a great number of carboxyl groups on its end site and these carboxyl ends provided anchoring points to the anti-RACK 1 antibodies. Anti-RACK 1 antibodies were covalently attached on the ITO electrode and recognized the RACK 1 antigens. Electrochemical characterizations were made by employing electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. Additionally, single frequency impedance method (SFI) was applied to follow the specific biointeraction between antibody and antigen. As a result of specific biointeraction, the designed immunosensor exhibited a wide linear detection range between 0.01 pg/mL and 2 pg/mL RACK 1 with a detection limit of 3.1 fg/mL. Scanning electron microscopy and atomic force microscopy analyses were employed for electrode surface morphology investigation. The designed RACK 1 biosensor had good repeatability (5.73 %, RSD), excellent reproducibility (2.5 %, RSD), long storage-stability and reusable property. In addition, the fabricated RACK 1 biosensor was applied to determine RACK 1 concentration in human serums and the recovery was ranging from 98.79%–100.22%. This work illustrated a new tool to construct a sensitive and low-cost disposable biosensor for applications in clinical monitoring. © 2020 Elsevier B.V.
Açıklama
Anahtar Kelimeler
Cancer biomarker, Conjugated polymer, Electrochemical biosensor, Electrochemical impedance spectroscopy, Receptor for activated C kinase 1, immobilized antibody, indium tin oxide, poly(3 thiophene acetic acid), polyethylene terephthalate, polymer, protein antibody, receptor for activated C kinase 1, unclassified drug, indium tin oxide, polymer, tin derivative, tumor marker, antigen detection, Article, atomic force microscopy, conjugation, controlled study, cyclic voltammetry, electrochemical detection, human, immobilization, impedance spectroscopy, limit of detection, limit of quantitation, priority journal, scanning electron microscopy, serum, electrochemical analysis, electrode, genetic procedures, immunoassay, neoplasm, reproducibility, Antibodies, Immobilized, Biomarkers, Tumor, Biosensing Techniques, Electrochemical Techniques, Electrodes, Humans, Immunoassay, Limit of Detection, Neoplasms, Polymers, Reproducibility of Results, Tin Compounds
Kaynak
Journal of Pharmaceutical and Biomedical Analysis
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
190
