Loss of Wwox contributes to cisplatin resistance in triple-negative breast cancer cells by modulating miR-182 and miR-214

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dc.contributor.authorBatar, Bahadir
dc.contributor.authorSerdal, Elif
dc.contributor.authorErdal, Berna
dc.contributor.authorOgul, Hasan
dc.date.accessioned2025-04-06T12:23:38Z
dc.date.available2025-04-06T12:23:38Z
dc.date.issued2024
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractBackground/aim: WW domain-containing oxidoreductase (WWOX) loss frequently occurs in triple-negative breast cancer (TNBC). WWOX loss enhances cisplatin resistance in TNBC patients. Although WWOX loss has an effect on the selection of a DNA repair pathway that contributes to enhanced mutagenesis, the downstream expression changes in resistant cancer cells have not been fully explored. This study aimed to investigate the potential role of microRNAs (miRNAs) in the regulation of cisplatin resistance in WWOX- deficient TNBC cells. Materials , methods: Transient transfections were performed to overexpress WWOX in MDA-MB-231 cells. WWOX-overexpressing MDA-MB-231 cells were determined by western blot. Expression profiling of the miRNA was assessed via real-time polymerase chain reaction. Results: miRNA expression profiling of WWOX-deficient and-sufficient MDA-MB-231 cells revealed that miR-182 upregulation and miR-214 downregulation were markedly positively associated with cisplatin resistance of WWOX-deficient MDA-MB-231 cells. An elevated expression of miR-182 and decreased expression of miR-214 may contribute to cisplatin resistance in WWOX-absent MDA- MB-231 cells by signaling pathway dysregulation of DNA repair/apoptosis/ protein kinase B (AKT). Conclusion: The results emphasize that WWOX deficiency promotes resistance to cisplatin in TNBC cells and the possible predicting biomarker of WWOX for resistance to cisplatin.
dc.description.sponsorshipTekirdagbreve; Namimath;k Kemal University Scientific Research Projects Committee [NKUBAP.02.GA.17.132]
dc.description.sponsorshipThis work was supported by the Tekirda & gbreve; Nam & imath;k Kemal University Scientific Research Projects Committee (project no: NKUBAP.02.GA.17.132) .
dc.identifier.doi10.55730/1300-0144.5891
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.issue5
dc.identifier.pmid39473747
dc.identifier.scopus2-s2.0-85208166513
dc.identifier.scopusqualityQ1
dc.identifier.trdizinid1279423
dc.identifier.urihttps://doi.org/10.55730/1300-0144.5891
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1279423
dc.identifier.urihttps://hdl.handle.net/20.500.11776/17093
dc.identifier.volume54
dc.identifier.wosWOS:001342117900029
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Medical Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250406
dc.subjectCisplatin resistance
dc.subjectmicroRNA
dc.subjecttriple-negative breast cancer
dc.subjectWWOX
dc.titleLoss of Wwox contributes to cisplatin resistance in triple-negative breast cancer cells by modulating miR-182 and miR-214
dc.typeArticle

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