Effects of Plant Growth Regulators on the Usage of In Vitro Stem Disc Culture for Mass Seedling Production in Kahramanmaraş Garlic (Allium sativum L.)

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dc.contributor.authorGülçebi, Ayşe
dc.contributor.authorAkıncı, İrfan Ersin
dc.date.accessioned2024-10-29T17:52:55Z
dc.date.available2024-10-29T17:52:55Z
dc.date.issued2023
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractAnatolia is one of the important production areas of garlic. Garlic production is conducted by using the head or the cloves inside the heads, which are actually the consumed part of the garlic, as propagation material. However, due to the use of cloves, which are the most valuable part of the market, as reproduction material, the profit is reduced by about 10%. The study aims to provide an alternative propagation material to reduce the losses resulting from this practice in local Kahramanmaraş garlic production. For this purpose, generating plantlets directly from stem discs of the garlic cloves in vitro and the effects of different growth regulators have been studied. For this, the plant growth regulators added to MS media as BAP (1.0, 1.5 and 2.0 mg l-1), GA3 (0.5, 1.0 and 1.5 mg l-1), 2-IP (0.75, 1.00 and 1.25 mg l-1); kinetin (1.0, 2.0 and 3.0 mg l-1) and TDZ (0.75, 1.00 and 1.25 mg l-1) were tested. In the study number of explants, number of infected explants, number of healthy explants, number of developed explants, healthy explant rate, developed explant rate, number of callused explants, callus growth rate, number of proliferated explants, proliferation rate, proliferation number, number of rooted explants, rooting rate and number of roots were investigated. However, shoot ratios, shoot numbers, and callus formation were the main focus. The highest rates of proliferation were found in 2-IP (53.8%, 45.5%, and 40.0% at 1.00, 0.75, and 1.25 mg l-1 dosages, respectively) and Kinetin (35.3% at 2.00 mg l-1). The maximum shoot number was reached with 2-IP at the dose of 1.00 mg l-1 as 1.9 shoot/explants. Kinetin at 3.00 mg l-1 and 2IP at 1.25 mg l-1 were the other successful applications with 1.8 shoots. This study indicated promising results to obtain plantlets directly from the clove’s stem discs and including them into seedling production for the mass production of garlic.
dc.identifier.doi10.33462/jotaf.1117827
dc.identifier.endpage560
dc.identifier.issn1302-7050
dc.identifier.issn2146-5894
dc.identifier.issue3en_US
dc.identifier.startpage550
dc.identifier.trdizinid1199721
dc.identifier.urihttps://doi.org/10.33462/jotaf.1117827
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1199721
dc.identifier.urihttps://hdl.handle.net/20.500.11776/13227
dc.identifier.volume20
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofTekirdağ Ziraat Fakültesi Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGA3
dc.subjectTDZ
dc.subjectBAP
dc.subjectGarlic
dc.subjectKinetin
dc.subject2-IP
dc.subjectStem disc culture
dc.titleEffects of Plant Growth Regulators on the Usage of In Vitro Stem Disc Culture for Mass Seedling Production in Kahramanmaraş Garlic (Allium sativum L.)
dc.typeArticle

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