A New Voltammetric Method for the Determination of Lercanidipine in Biological Samples
Yükleniyor...
Dosyalar
Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Slovensko Kemijsko Drustvo
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Electrochemical behavior and adsorption-diffusion properties of lercanidipine (LCN) on a glassy carbon electrode (GCE) were investigated in a mixture of ethanol-Britton Robinson buffer (BR) using voltammetric methods. From experimental results LCN was found to be reduced irreversibly via a single four-electron process controlled mainly by diffusion with some adsorption contribution at about -0.65 V (vs. Ag/AgCl reference electrode). Therefore, a new, accurate, rapid, selective and simple square-wave cathodic adsorptive stripping voltammetric (SWCAdSV) method could be developed for direct determination of LCN in pharmaceutical preparations, spiked human urine and spiked human serum samples without time-consuming steps prior to drug assay. The peak current of the reduction wave linearly changed with the concentration of LCN in the concentration range between 4.0 x 10(-8) molL(-1) and 7.6 x 10(-6) molL(-1) in two different regions where optimum preconcentration potential and optimum preconcentration time were applied as -0.20 V and 90 s, respectively. The limit of detection (LOD) and the limit of quantitation (LOQ) values were found to be 2 x 10(-8) molL(-1) (0.01 mgL(-1)) and 6 x 10(-8) molL(-1) (0.04 mgL(-1)), respectively. The method was applied to determine the content of LCN in commercial pharmaceutical preparation, spiked human serum and spiked human urine. The method was found to be highly accurate and precise, having a relative standard deviation of less than 10% in all applications.
Açıklama
Anahtar Kelimeler
Electrochemistry, Cyclic voltammetry, Square-wave adsorptive stripping voltammetry, Lercanidipine, Antihypertensive drug, Biological samples (spiked), Performance Liquid-Chromatography, Calcium-Channel Antagonists, Mass-Spectrometry Method, Human Plasma, Spectrophotometric Determination, 5 1,4-Dihydropyridines, Dosage Forms, Hplc Method, Hydrochloride, Impurities
Kaynak
Acta Chimica Slovenica
WoS Q Değeri
Q2
Scopus Q Değeri
Q3
Cilt
58
Sayı
4
