A new immobilization procedure for development of an electrochemical immunosensor for parathyroid hormone detection based on gold electrodes modified with 6-mercaptohexanol and silane

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dc.authorid0000-0002-3153-1328
dc.authorscopusid56688584400
dc.authorscopusid56688569500
dc.authorscopusid6506764918
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.authorwosidKaraboga, Munteha Nur Sonuc/ABA-6419-2020
dc.contributor.authorSayıklı Şimşek, Çiğdem
dc.contributor.authorSonuç Karaboğa, Münteha Nur
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:15:41Z
dc.date.available2022-05-11T14:15:41Z
dc.date.issued2015
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.departmentYüksekokullar, Sağlık Yüksekokulu, Beslenme ve Diyetetik Bölümü
dc.description.abstractFabrication of a new electrochemical impedance-based biosensor for the analysis of parathyroid hormone (PTH), using self-assembled monolayers (SAMs) of mercaptohexanol and (3-Aminopropyl) triethoxysilane on gold electrodes, was investigated for the first time in the field. Anti-PTH was used as a biorecognition element. To monitor immobilization processes in the biosensor fabrication, cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM) techniques were successfully operated. CV and EIS techniques were also used in quantification of PTH. Energy-dispersive X-ray analysis (EDAX) was also applied to identify surface modifications. Fabrication and working parameters of the biosensor were optimized. Moreover, Kramers-Kronig transformations were performed for validation of obtained EIS data in all steps of biosensor fabrication. The linear PTH detection range of the presented biosensor was 10-50 pg/mL PTH. The chrono-impedance technique for real-time monitoring of PTH binding was also implemented. The biosensor has exhibited good repeatability (with a correlation) and reproducibility. Finally, artificial serum samples spiked with known concentrations of PTH were analyzed by the proposed biosensor. To demonstrate the feasibility of the biosensor in practical analysis, real human serum samples and the artificial serum samples were analyzed. (C) 2015 Elsevier B.V. All rights reserved.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113 Z 678]
dc.description.sponsorshipSupport from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 113 Z 678) is greatly acknowledged. Also we are grateful to Namik Kemal University Scientific and Technological Research Centre (NABILTEM).
dc.identifier.doi10.1016/j.talanta.2015.06.010
dc.identifier.endpage218
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid26452812
dc.identifier.scopus2-s2.0-84931292123
dc.identifier.scopusqualityQ1
dc.identifier.startpage210
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2015.06.010
dc.identifier.urihttps://hdl.handle.net/20.500.11776/6028
dc.identifier.volume144
dc.identifier.wosWOS:000363346200029
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSayıklı Şimşek, Çiğdem
dc.institutionauthorSonuç Karaboğa, Münteha Nur
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofTalanta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectParathyroid hormone
dc.subjectBiosensor
dc.subject6-Mercaptohexanol
dc.subjectSilane
dc.subjectSingle frequency impedance
dc.subjectMortality
dc.subjectCalcium
dc.subjectRisk
dc.titleA new immobilization procedure for development of an electrochemical immunosensor for parathyroid hormone detection based on gold electrodes modified with 6-mercaptohexanol and silane
dc.typeArticle

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