Fabrication of a highly sensitive disposable immunosensor based on indium tin oxide substrates for cancer biomarker detection

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Küçük Resim

Tarih

2014

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Academic Press Inc Elsevier Science

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Anti-HER-3 antibody was used for the first time in a disposable immunosensor based on indium tin oxide (ITO) substrate for HER-3 quantification. Anti-HER-3 was immobilized onto ITO substrate by 3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde. This highly sensitive immunosensor was capable of detecting concentrations of HER-3 down to the femtogram/ml level by investigating changes in the charge transfer resistance (R-ct) using electrochemical impedance spectroscopy (EIS). Construction of ITO layers was carefully investigated using a broad range of techniques such as voltammetry, EIS, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Meanwhile, in an immunosensor system, the single frequency impedance technique was first used for characterization of interaction between HER-3 and anti-HER-3. Eventually, the proposed ITO-based immunosensor was applied to artificial serum samples spiked with HER-3. (C) 2013 Elsevier Inc. All rights reserved.

Açıklama

Anahtar Kelimeler

Cancer biomarkers, Biosensor, HER-3, ITO, Single frequency impedance, Breast-Cancer, Expression, Her3, Survival, Prognosis, C-Erbb-3, Protein

Kaynak

Analytical Biochemistry

WoS Q Değeri

Q2

Scopus Q Değeri

Q3

Cilt

446

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Künye