A comparison between LP(GMA) and CLP(GMA) polymer composites as an immobilization matrix for biosensing applications: A model immunosensor for IL 1a

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dc.authorscopusid57194273220
dc.authorscopusid6506764918
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:07:22Z
dc.date.available2022-05-11T14:07:22Z
dc.date.issued2019
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractA comparative study was made by using two matrices (Linear poly(glycidyl methacrylate) (GMA) polymer (LP(GMA)) and carbon black-polyvinylidene fluoride (PVDF)-LP(GMA) composite CLP(GMA)) as immobilization platforms for anti-IL 1? antibody on the development of impedimetric immunosensor for IL 1? biomarker determination. These materials were spin coated onto the clean ITO electrode surface separately and used for IL 1? immunosensor fabrication. Carbon black is utilized as a conductive material and has been employed over the last few decades for electrochemical biosensors development. The using of CLP(GMA) composite as an interface material shows fast electron transfer, when compared to LP(GMA) modified ITO electrode. This comparative study investigated the efficacy of carbon black for impedimetric biosensing. Anti-IL 1? antibodies were utilized as bioreceptors and bound to epoxy groups of GMA polymer. For electrochemical characterizations, Electrochemical Impedance Spectroscopy, Cyclic Voltammetry and Single Frequency Impedance methods were employed. Furthermore, to follow the antibody attachment on the modified ITO substrates, Fourier Transform Infrared Spectroscopy and Raman spectral techniques were utilized. The morphological characterizations of immunosensor construction stages were carried out by Scanning Electron Microscopy and Atomic Force Microscopy analyses. The sensitive and label-free technique of EIS was used for quantification of IL 1? concentration. Under optimum experimental conditions, the immunosensor had a good linear relationship between impedance values and the IL 1? concentrations ranging from 0.01 to 2 pg/mL and from 0.01 to 3 pg/mL for LP(GMA) and CLP(GMA) modified ITO electrodes, respectively. The results confirmed that CLP(GMA) used in the preparation of the immunosensor illustrated improvements in immunosensor performance, comparing to the LP(GMA) modified immunosensor. Additionally, the immunosensor was successfully applied to evaluate human serum and saliva samples. The obtained results from these samples illustrated the feasibility of the immunosensor for clinical diagnosis in complex biological samples. Also, a simple and low-cost approach was attempted for development of immunosensor with remarkable performance characteristics. © 2019 Elsevier B.V.
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAK: 113 Z 678
dc.description.sponsorshipThe financial support from T?BITAK (The Scientific and Technological Research Council of Turkey, Project number: 113 Z 678) is gratefully acknowledged.
dc.description.sponsorshipThe financial support from TÜBITAK (The Scientific and Technological Research Council of Turkey, Project number: 113 Z 678 ) is gratefully acknowledged.
dc.identifier.doi10.1016/j.aca.2019.05.049
dc.identifier.endpage139
dc.identifier.issn0003-2670
dc.identifier.pmid31307701
dc.identifier.scopus2-s2.0-85066075206
dc.identifier.scopusqualityQ1
dc.identifier.startpage129
dc.identifier.urihttps://doi.org/10.1016/j.aca.2019.05.049
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5087
dc.identifier.volume1077
dc.identifier.wosWOS:000475299800009
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Elif Burcu
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofAnalytica Chimica Acta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDisposable ITO electrode
dc.subjectElectrochemical impedance spectroscopy
dc.subjectInterleukin 1?
dc.subjectpoly(glycidyl methacrylate) polymer
dc.subjectAcrylic monomers
dc.subjectAntibodies
dc.subjectAtomic force microscopy
dc.subjectBody fluids
dc.subjectCarbon black
dc.subjectCoated materials
dc.subjectConductive materials
dc.subjectCyclic voltammetry
dc.subjectDiagnosis
dc.subjectElectrochemical electrodes
dc.subjectFluorine compounds
dc.subjectFourier transform infrared spectroscopy
dc.subjectImmunosensors
dc.subjectMatrix algebra
dc.subjectPolymer matrix composites
dc.subjectScanning electron microscopy
dc.subjectElectrochemical biosensor
dc.subjectElectrochemical characterizations
dc.subjectImpedimetric immunosensors
dc.subjectInterleukin-1
dc.subjectITO electrodes
dc.subjectMorphological characterization
dc.subjectPerformance characteristics
dc.subjectPoly(glycidyl methacrylate)
dc.subjectElectrochemical impedance spectroscopy
dc.subjectblack carbon
dc.subjectcarbon black polyvinylidene fluoride linear poly(glycidyl methacrylate)
dc.subjectinterleukin 1 antibody
dc.subjectinterleukin 1alpha
dc.subjectlinear poly(glycidyl methacrylate)
dc.subjectpolymer
dc.subjectpolyvinylidene fluoride
dc.subjectunclassified drug
dc.subjectIL1A protein, human
dc.subjectimmobilized antibody
dc.subjectindium tin oxide
dc.subjectinterleukin 1alpha
dc.subjectpolyglycidyl methacrylate
dc.subjectpolymethacrylic acid derivative
dc.subjectpolyvinyl derivative
dc.subjecttin derivative
dc.subjectantigen binding
dc.subjectArticle
dc.subjectatomic force microscopy
dc.subjectblood sampling
dc.subjectcomparative study
dc.subjectcyclic potentiometry
dc.subjectelectrochemical detection
dc.subjectelectron transport
dc.subjectFourier transform infrared spectroscopy
dc.subjecthuman
dc.subjectimpedance spectroscopy
dc.subjectpriority journal
dc.subjectprotein immobilization
dc.subjectRaman spectrometry
dc.subjectsaliva analysis
dc.subjectscanning electron microscopy
dc.subjectblood
dc.subjectchemistry
dc.subjectdevices
dc.subjectelectrochemical analysis
dc.subjectelectrode
dc.subjectgenetic procedures
dc.subjectimmunoassay
dc.subjectimmunology
dc.subjectlimit of detection
dc.subjectprocedures
dc.subjectsaliva
dc.subjectsoot
dc.subjectAntibodies, Immobilized
dc.subjectBiosensing Techniques
dc.subjectElectrochemical Techniques
dc.subjectElectrodes
dc.subjectHumans
dc.subjectImmunoassay
dc.subjectInterleukin-1alpha
dc.subjectLimit of Detection
dc.subjectPolymethacrylic Acids
dc.subjectPolyvinyls
dc.subjectSaliva
dc.subjectSoot
dc.subjectTin Compounds
dc.titleA comparison between LP(GMA) and CLP(GMA) polymer composites as an immobilization matrix for biosensing applications: A model immunosensor for IL 1a
dc.typeArticle

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