A comparison between LP(GMA) and CLP(GMA) polymer composites as an immobilization matrix for biosensing applications: A model immunosensor for IL 1a
Yükleniyor...
Dosyalar
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier B.V.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A comparative study was made by using two matrices (Linear poly(glycidyl methacrylate) (GMA) polymer (LP(GMA)) and carbon black-polyvinylidene fluoride (PVDF)-LP(GMA) composite CLP(GMA)) as immobilization platforms for anti-IL 1? antibody on the development of impedimetric immunosensor for IL 1? biomarker determination. These materials were spin coated onto the clean ITO electrode surface separately and used for IL 1? immunosensor fabrication. Carbon black is utilized as a conductive material and has been employed over the last few decades for electrochemical biosensors development. The using of CLP(GMA) composite as an interface material shows fast electron transfer, when compared to LP(GMA) modified ITO electrode. This comparative study investigated the efficacy of carbon black for impedimetric biosensing. Anti-IL 1? antibodies were utilized as bioreceptors and bound to epoxy groups of GMA polymer. For electrochemical characterizations, Electrochemical Impedance Spectroscopy, Cyclic Voltammetry and Single Frequency Impedance methods were employed. Furthermore, to follow the antibody attachment on the modified ITO substrates, Fourier Transform Infrared Spectroscopy and Raman spectral techniques were utilized. The morphological characterizations of immunosensor construction stages were carried out by Scanning Electron Microscopy and Atomic Force Microscopy analyses. The sensitive and label-free technique of EIS was used for quantification of IL 1? concentration. Under optimum experimental conditions, the immunosensor had a good linear relationship between impedance values and the IL 1? concentrations ranging from 0.01 to 2 pg/mL and from 0.01 to 3 pg/mL for LP(GMA) and CLP(GMA) modified ITO electrodes, respectively. The results confirmed that CLP(GMA) used in the preparation of the immunosensor illustrated improvements in immunosensor performance, comparing to the LP(GMA) modified immunosensor. Additionally, the immunosensor was successfully applied to evaluate human serum and saliva samples. The obtained results from these samples illustrated the feasibility of the immunosensor for clinical diagnosis in complex biological samples. Also, a simple and low-cost approach was attempted for development of immunosensor with remarkable performance characteristics. © 2019 Elsevier B.V.
Açıklama
Anahtar Kelimeler
Disposable ITO electrode, Electrochemical impedance spectroscopy, Interleukin 1?, poly(glycidyl methacrylate) polymer, Acrylic monomers, Antibodies, Atomic force microscopy, Body fluids, Carbon black, Coated materials, Conductive materials, Cyclic voltammetry, Diagnosis, Electrochemical electrodes, Fluorine compounds, Fourier transform infrared spectroscopy, Immunosensors, Matrix algebra, Polymer matrix composites, Scanning electron microscopy, Electrochemical biosensor, Electrochemical characterizations, Impedimetric immunosensors, Interleukin-1, ITO electrodes, Morphological characterization, Performance characteristics, Poly(glycidyl methacrylate), Electrochemical impedance spectroscopy, black carbon, carbon black polyvinylidene fluoride linear poly(glycidyl methacrylate), interleukin 1 antibody, interleukin 1alpha, linear poly(glycidyl methacrylate), polymer, polyvinylidene fluoride, unclassified drug, IL1A protein, human, immobilized antibody, indium tin oxide, interleukin 1alpha, polyglycidyl methacrylate, polymethacrylic acid derivative, polyvinyl derivative, tin derivative, antigen binding, Article, atomic force microscopy, blood sampling, comparative study, cyclic potentiometry, electrochemical detection, electron transport, Fourier transform infrared spectroscopy, human, impedance spectroscopy, priority journal, protein immobilization, Raman spectrometry, saliva analysis, scanning electron microscopy, blood, chemistry, devices, electrochemical analysis, electrode, genetic procedures, immunoassay, immunology, limit of detection, procedures, saliva, soot, Antibodies, Immobilized, Biosensing Techniques, Electrochemical Techniques, Electrodes, Humans, Immunoassay, Interleukin-1alpha, Limit of Detection, Polymethacrylic Acids, Polyvinyls, Saliva, Soot, Tin Compounds
Kaynak
Analytica Chimica Acta
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
1077
