Electrochemical Immunosensor for Detection of CCR4 Cancer Biomarker in Human Serum: An Alternative Strategy for Modification of Disposable ITO Electrode

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dc.authorscopusid57194273220
dc.authorscopusid55571487900
dc.authorscopusid6506764918
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:07:25Z
dc.date.available2022-05-11T14:07:25Z
dc.date.issued2021
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractHerein, a new strategy for the fabrication of a sensitive immunosensor capable of determination of CC Chemokine receptor 4 (CCR4) in complex serum samples is developed through the polymer modification on the disposable indium tin oxide electrode. Anti-CCR4 antibodies, which are utilized as sensing biomolecules, are covalently attached on the succinimide groups of polypyrrole polymer (PPyr-CSsg). The constructed immunosensor illustrates promising performances for the quantification of CCR4 antigen, with a linear detection range of 0.024–12 pg mL?1 and a low detection limit of 7.3 fg mL?1, calculated at a signal-to-noise ratio of 3. In addition, the impedimetric immunosensor displays a very successful analytical performance in terms of sensitivity, selectivity, repeatability, reproducibility, and long-term stability as well as successful applicability for the accurate quantification of CCR4 in human serum samples. The constructed immunosensor is successfully used for quantification of CCR4 antigen in human serums. In addition, the immunosensor displays only 27.54% loss in its initial signal after nine weeks storage at 4 °C. Moreover, the fabricated immunosensor is economical, highly sensitive, and selective for CCR4 antigen detection, and suitable for potential application in clinical diagnosis. © 2020 Wiley-VCH GmbH
dc.description.sponsorshipÇanakkale Onsekiz Mart Üniversitesi, ÇOMÜ: FUK?2018?1396
dc.description.sponsorshipThis research was supported by The Scientific and Technological Research Council of Çanakkale Onsekiz Mart University, ÇOMÜ?BAP (Grant No. FUK?2018?1396).
dc.description.sponsorshipThis research was supported by The Scientific and Technological Research Council of ?anakkale Onsekiz Mart University, ?OM?-BAP (Grant No. FUK-2018-1396).
dc.identifier.doi10.1002/mabi.202000267
dc.identifier.issn1616-5187
dc.identifier.issue2en_US
dc.identifier.pmid33108068
dc.identifier.scopus2-s2.0-85093976722
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1002/mabi.202000267
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5099
dc.identifier.volume21
dc.identifier.wosWOS:000583873700001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Muhammet
dc.institutionauthorAydın, Elif Burcu
dc.language.isoen
dc.publisherWiley-VCH Verlag
dc.relation.ispartofMacromolecular Bioscience
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCC Chemokine receptor 4
dc.subjectelectrochemical impedance spectroscopy
dc.subjectimmunosensor
dc.subjectpolypyrrole polymer containing succinimide groups
dc.subjectAntigens
dc.subjectChemical detection
dc.subjectDiagnosis
dc.subjectImmunosensors
dc.subjectPolypyrroles
dc.subjectSignal to noise ratio
dc.subjectTin oxides
dc.subjectAccurate quantifications
dc.subjectAnalytical performance
dc.subjectElectrochemical immunosensors
dc.subjectImpedimetric immunosensors
dc.subjectIndium tin oxide electrodes
dc.subjectLinear detection ranges
dc.subjectLong term stability
dc.subjectPolymer modifications
dc.subjectElectrochemical electrodes
dc.subjectchemokine receptor CCR4
dc.subjectglobular protein
dc.subjectindium tin oxide
dc.subjectsuccinimide
dc.subjecttumor marker
dc.subjectchemokine receptor CCR4
dc.subjectindium tin oxide
dc.subjecttin derivative
dc.subjecttumor marker
dc.subjectantibody blood level
dc.subjectantigen antibody complex
dc.subjectantigen detection
dc.subjectArticle
dc.subjectcontrolled study
dc.subjectcovalent bond
dc.subjectelectric conductivity
dc.subjectelectron transport
dc.subjectfast electron radiation
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectlimit of detection
dc.subjectlimit of quantitation
dc.subjectmeasurement repeatability
dc.subjectoxidative coupling
dc.subjectreproducibility
dc.subjectscanning electron microscopy
dc.subjectsignal noise ratio
dc.subjectsurface property
dc.subjectatomic force microscopy
dc.subjectblood
dc.subjectchemistry
dc.subjectelectrochemical analysis
dc.subjectelectrode
dc.subjectgenetic procedures
dc.subjectimpedance spectroscopy
dc.subjectinfrared spectroscopy
dc.subjectRaman spectrometry
dc.subjectBiomarkers, Tumor
dc.subjectBiosensing Techniques
dc.subjectDielectric Spectroscopy
dc.subjectElectrochemical Techniques
dc.subjectElectrodes
dc.subjectHumans
dc.subjectMicroscopy, Atomic Force
dc.subjectReceptors, CCR4
dc.subjectReproducibility of Results
dc.subjectSpectroscopy, Fourier Transform Infrared
dc.subjectSpectrum Analysis, Raman
dc.subjectTin Compounds
dc.titleElectrochemical Immunosensor for Detection of CCR4 Cancer Biomarker in Human Serum: An Alternative Strategy for Modification of Disposable ITO Electrode
dc.typeArticle

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