Electrochemical Immunosensor for Detection of CCR4 Cancer Biomarker in Human Serum: An Alternative Strategy for Modification of Disposable ITO Electrode
Yükleniyor...
Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-VCH Verlag
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Herein, a new strategy for the fabrication of a sensitive immunosensor capable of determination of CC Chemokine receptor 4 (CCR4) in complex serum samples is developed through the polymer modification on the disposable indium tin oxide electrode. Anti-CCR4 antibodies, which are utilized as sensing biomolecules, are covalently attached on the succinimide groups of polypyrrole polymer (PPyr-CSsg). The constructed immunosensor illustrates promising performances for the quantification of CCR4 antigen, with a linear detection range of 0.024–12 pg mL?1 and a low detection limit of 7.3 fg mL?1, calculated at a signal-to-noise ratio of 3. In addition, the impedimetric immunosensor displays a very successful analytical performance in terms of sensitivity, selectivity, repeatability, reproducibility, and long-term stability as well as successful applicability for the accurate quantification of CCR4 in human serum samples. The constructed immunosensor is successfully used for quantification of CCR4 antigen in human serums. In addition, the immunosensor displays only 27.54% loss in its initial signal after nine weeks storage at 4 °C. Moreover, the fabricated immunosensor is economical, highly sensitive, and selective for CCR4 antigen detection, and suitable for potential application in clinical diagnosis. © 2020 Wiley-VCH GmbH
Açıklama
Anahtar Kelimeler
CC Chemokine receptor 4, electrochemical impedance spectroscopy, immunosensor, polypyrrole polymer containing succinimide groups, Antigens, Chemical detection, Diagnosis, Immunosensors, Polypyrroles, Signal to noise ratio, Tin oxides, Accurate quantifications, Analytical performance, Electrochemical immunosensors, Impedimetric immunosensors, Indium tin oxide electrodes, Linear detection ranges, Long term stability, Polymer modifications, Electrochemical electrodes, chemokine receptor CCR4, globular protein, indium tin oxide, succinimide, tumor marker, chemokine receptor CCR4, indium tin oxide, tin derivative, tumor marker, antibody blood level, antigen antibody complex, antigen detection, Article, controlled study, covalent bond, electric conductivity, electron transport, fast electron radiation, human, human tissue, limit of detection, limit of quantitation, measurement repeatability, oxidative coupling, reproducibility, scanning electron microscopy, signal noise ratio, surface property, atomic force microscopy, blood, chemistry, electrochemical analysis, electrode, genetic procedures, impedance spectroscopy, infrared spectroscopy, Raman spectrometry, Biomarkers, Tumor, Biosensing Techniques, Dielectric Spectroscopy, Electrochemical Techniques, Electrodes, Humans, Microscopy, Atomic Force, Receptors, CCR4, Reproducibility of Results, Spectroscopy, Fourier Transform Infrared, Spectrum Analysis, Raman, Tin Compounds
Kaynak
Macromolecular Bioscience
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
21
Sayı
2
