Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis

Basit Öğe Kaydı
dc.contributor.authorCanbolat, Fadime
dc.contributor.authorKenanoglu, Nihan Akıncı
dc.contributor.authorYuksel, Tugba Nurcan
dc.contributor.authorBerber, Ahmet Ali
dc.date.accessioned2024-10-29T17:50:01Z
dc.date.available2024-10-29T17:50:01Z
dc.date.issued2023
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractValproic acid (VPA) is among the most commonly used antiepileptic drugs in childhood and adult epilepsy. Although VPA is well tolerated, it can cause life-threatening side effects. VPA has toxic and genotoxic effects. Antioxidants can reverse drugs' toxic and genotoxic effects. Therefore, our study aimed to evaluate the antigenotoxic protective effect of quercetin (QUE) against VPA genotoxicity by in vitro comet assay analysis method. Comet assay analysis was performed in five different groups. Group I; negative control (Sterile H2O), Group II; positive control (H2O2), Group III; VPA was applied in four different dose ranges, Group IV; QUE was applied in four different dose ranges, Group V; For the simultaneous combined administration of VPA and QUE, three different doses of VPA + four different doses of QUE were administered. Low-dose administration of QUE was more effective in ameliorating the damage caused by low-dose VPA (62.5 ?g/ml) administration. It is seen that the genotoxic damage caused by the application of 125 ?g/ml VPA can be eliminated by QUE at all doses. It was determined that different doses of QUE exhibited a significant antigenotoxic effect against damage caused by 125 µg/mL VPA (P<0.05). In our study, the curative effect of QUE on DNA damage was determined by in vitro comet analysis. Our analysis results showed that QUE ameliorates VPA-induced genetic damage.
dc.identifier.doi10.16984/saufenbilder.1339199
dc.identifier.endpage1196
dc.identifier.issn1301-4048
dc.identifier.issn2147-835X
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85187305572
dc.identifier.startpage1185
dc.identifier.trdizinid1212392
dc.identifier.urihttps://doi.org/10.16984/saufenbilder.1339199
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1212392
dc.identifier.urihttps://hdl.handle.net/20.500.11776/12696
dc.identifier.volume27
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofSakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectIn vitro
dc.subjectquercetin
dc.subjectcomet assay
dc.subjectValproic acid
dc.titleEvaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis
dc.typeArticle

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu