Dietary intake of Spirulina platensis alters HSP70 gene expression profiles in the brain of rats in an experimental model of mixed stress

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dc.authoridKOSELI, EDA/0000-0002-4812-4024
dc.authoridArdicli, Sena/0000-0003-2758-5945
dc.authorwosidKOSELI, EDA/GRJ-2026-2022
dc.contributor.authorArdıçlı, Sena
dc.contributor.authorSeyidoğlu, Nilay
dc.contributor.authorKöseli, Eda
dc.contributor.authorGurbanli, Rovshan
dc.contributor.authorAydın, Cenk
dc.date.accessioned2023-05-06T17:23:37Z
dc.date.available2023-05-06T17:23:37Z
dc.date.issued2022
dc.departmentFakülteler, Veteriner Fakültesi, Temel Bilimler Bölümü, Fizyoloji Ana Bilim Dalı
dc.description.abstractSpirulina platensis has gradually gained more attention for its therapeutic, antioxidant, and anti-inflammatory potential worldwide. However, the current molecular knowledge about the effects of spirulina on stress-related genes is rather limited. The effects of dietary intake of spirulina on the HSP70 gene expression were assessed in a controlled in vivo experimental design. Moreover, alterations in serum corticosterone levels, IL-2, IL-4, IFN-gamma, triglyceride, ALT, AST, relative gene expression values, and the correlations between them were evaluated. A total of 36 rats were divided into four groups: control group, stress-only group, spirulina group, and spirulina+stress group. To control the dose administration, S. platensis was applied by a gastric gavage in stress groups. Crowded environment stress and hosting alone stress were applied to the stress-only group and spirulina + stress group. RNA was extracted from brain samples using TRlpure and the relative gene expression assessment was performed using Roche-LightCycler-480-II real-time PCR-System. Gene expression values were remarkably different among the four experimental groups. The differences between stress-only and the spirulina groups were statistically significant (P<0.05). The correlation between the HSP70 gene expression and the IFN-gamma was found to be statistically significant (P<0.05; r=0.50). Results indicate a novel effect of spirulina on the HSP70 expression related to the stress-response. Data presented in this study may be useful for further studies to define not only the molecular genetic aspects through dietary S. platensis but also the effects of spirulina on stress-response and animal welfare.
dc.description.sponsorshipYoK (Council of Higher Education) 100/200 Doctoral Research Fellowship Programme; TuBTAK (The Scientific and Technological Research Council of Turkey)
dc.description.sponsorshipThe data on some parts of the research has been presented as an oral presentation at the 2nd International Eurasian Conference on Science, Engineering and Technology (EurasianSciEnTech 2020), Gaziantep, Turkey. Eda Koseli was supported by YoK (Council of Higher Education) 100/200 Doctoral Research Fellowship Programme and TuBTAK (The Scientific and Technological Research Council of Turkey) 2211-C (National Ph.D. Scholarship Programme in the Priority Fields in Science and Technology). The authors are grateful to Dr Ozge Ardicli for her support.
dc.identifier.doi10.1007/s12041-022-01388-5
dc.identifier.issn0022-1333
dc.identifier.issn0973-7731
dc.identifier.issue2en_US
dc.identifier.pmid36330789
dc.identifier.scopus2-s2.0-85140641024
dc.identifier.scopusqualityQ4
dc.identifier.urihttps://doi.org/10.1007/s12041-022-01388-5
dc.identifier.urihttps://hdl.handle.net/20.500.11776/12195
dc.identifier.volume101
dc.identifier.wosWOS:000873842100002
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSeyidoğlu, Nilay
dc.language.isoen
dc.publisherIndian Acad Sciences
dc.relation.ispartofJournal Of Genetics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectgene expression
dc.subjectHSP70 gene
dc.subjectreal-time polymerase chain reaction
dc.subjectSpirulina (Arthrospira) platensis
dc.subjectstress
dc.subjectHeat-Shock Factor
dc.subjectC-Phycocyanin
dc.subjectOxidative Stress
dc.subjectMessenger-Rna
dc.subjectSupplementation
dc.subjectLipopolysaccharide
dc.subjectInflammation
dc.subjectAntioxidant
dc.subjectResistance
dc.subjectImmunity
dc.titleDietary intake of Spirulina platensis alters HSP70 gene expression profiles in the brain of rats in an experimental model of mixed stress
dc.typeArticle

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