AuNPs modified, disposable, ITO based biosensor: Early diagnosis of heat shock protein 70
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Date
2016
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier Advanced Technology
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
This paper describes a novel, simple, and disposable immunosensor based on indium-tin oxide (ITO) sheets modified with gold nanoparticles to sensitively analyze heat shock protein 70 (HSP70), a potential biomarker that could be evaluated in diagnosis of some carcinomas. Disposable ITO coated Polyethylene terephthalate (PET) electrodes were used and modified with gold nanoparticles in order to construct the biosensors. Optimization and characterization steps were analyzed by electrochemical techniques such as electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Surface morphology of the biosensor was also identified by electrochemical methods, scanning electron microscopy (SEM), and atomic force microscopy (AFM). To interpret binding characterization of HSP70 to anti-HSP70 single frequency impedance method was successfully operated. Moreover, the proposed HSP70 immunosensor acquired good stability, repeatability, and reproducibility. Ultimately, proposed biosensor was introduced to real human serum samples to determine HSP70 sensitively and accurately. (C) 2015 Elsevier B.V. All rights reserved.
Description
Keywords
Heat shock proteins, HSP70, ITO, Gold nanoparticles, Immunosensor, Heat-Shock Proteins, Hsp70, Cancer, Expression, Immunosensor, Chaperones, Nanoparticles, Sensors
Journal or Series
Biosensors & Bioelectronics
WoS Q Value
Q1
Scopus Q Value
Q1
Volume
84
