Molecular Determination of the Presence of Some Grapevine Viruses and Phylogenetic analyses of Grapevine Virus A in Tokat Province Grapevine Area

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dc.contributor.authorGüvenateş, Burak
dc.contributor.authorTopkaya, Şerife
dc.date.accessioned2024-10-29T17:52:55Z
dc.date.available2024-10-29T17:52:55Z
dc.date.issued2023
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractGrapevine (Vitis spp.) is one of the major fruit crop with high socioeconomic importance for Turkey. In vineyards, many harmful organism, especially virus infections, weaken the plant and lead to decreases in yield and quality, so it takes the lead in quarantine and certification. This study was carried out to determine some viral agents that cause yield loss in vines produced in Tokat, where viticulture is very important. Samples were collected from young leaves and one-year-old shoots of grapevines showing virus symptoms from some vineyard areas in Tokat Center and its districts. Collected 189 grapevine samples were subjected to the RT-PCR test, which is a molecular method using virus-specific primers, to detect the presence of Grapevine pinot gris virus (GPGV), Grapevine virus A (GVA), Strawberry latent ringspot virus (SLRSV). Out of a total of 189 plant samples, 80 (42.32%) of GVA, 3 (1.58%) of GPGV were detected and SLRSV (0%) was not detected. More than one virus was found in 2 (1.05%) of 189 tested samples. It was determined that the most common virus was GVA, the least detected virus was GPGV in plant samples collected from Tokat Center and its districts. Bidirectional sequence analysis of RT-PCR products of GVA-infected isolates were performed and phylogenetic analyzes were done by comparing them with reference isolates after they were aligned with the MEGAX computer program. Based on phylogenetic analysis studies, GVA showed differential branching with isolates registered in GenBank and isolates obtained in the study. GVA-infected isolates showed similarity with reference isolates at rates of 92-94%. In this study, molecular analysis of Turkish GVA isolates was performed. This molecular information is important as it will shed light on future studies.
dc.identifier.doi10.33462/jotaf.1124089
dc.identifier.endpage585
dc.identifier.issn1302-7050
dc.identifier.issn2146-5894
dc.identifier.issue3en_US
dc.identifier.startpage574
dc.identifier.trdizinid1199723
dc.identifier.urihttps://doi.org/10.33462/jotaf.1124089
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1199723
dc.identifier.urihttps://hdl.handle.net/20.500.11776/13231
dc.identifier.volume20
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofTekirdağ Ziraat Fakültesi Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectVitis vinifera
dc.subjectTokat
dc.subjectRT-PCR
dc.subjectPhylogenetic analysis
dc.subjectGrapevine virus A
dc.subjectStrawberry latent ringspot virus
dc.subjectGrapevine pinot gris virus
dc.titleMolecular Determination of the Presence of Some Grapevine Viruses and Phylogenetic analyses of Grapevine Virus A in Tokat Province Grapevine Area
dc.typeArticle

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