A Highly Selective Poly(thiophene)-graft-Poly(methacrylamide) Polymer Modified ITO Electrode for Neuron Specific Enolase Detection in Human Serum

Basit Öğe Kaydı
dc.authorscopusid55571487900
dc.authorscopusid57194273220
dc.authorscopusid6506764918
dc.contributor.authorAydın, Muhammet
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:07:23Z
dc.date.available2022-05-11T14:07:23Z
dc.date.issued2019
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractIn this study, an impedimetric immunosensor based on polymer poly(thiophene)-graft-poly(methacrylamide) polymer (P(Thi-g-MAm)) modified indium tin oxide (ITO) electrode is developed for the detection of the Neuron Specific Enolase (NSE) cancer biomarker. First, the P(Thi-g-MAm) polymer is synthesized and coated on the ITO electrode by using a spin-coating technique. P(Thi-g-MAm) polymer acts as an immobilization platform for immobilization of NSE-specific monoclonal antibodies. Anti-NSE antibodies are utilized as biosensing molecules and they bind to the amino groups of P(Thi-g-Mam) polymer via glutaraldehyde cross-linking. Spin-coating technique is employed for bioelectrode fabrication and this technique provides a thin and uniform film on the ITO electrode surface. This bioelectrode fabrication technique is simple and it generates a suitable platform for large-scale loadings of anti-NSE antibodies. This immunosensor exhibits a wide linear detection range from 0.02 to 4 pg mL?1 and with an ultralow detection limit of 6.1 fg mL?1. It reveals a good long-term stability (after 8 weeks, 78% of its initial activity), an excellent reproducibility (1.29% of relative standard deviation (RSD)), a good repeatability (5.55% of RSD), and a high selectivity. In addition, the developed immunosensor is proposed as a robust diagnostic tool for the clinical detection of NSE and other cancer biomarkers. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
dc.description.sponsorshipDS.18.180, NKUBAP.00
dc.description.sponsorshipThis research was supported The Scientific and Technological Research Council of by Tekirdag? Nam?k Kemal University, NKU-BAP (Grant number: NKUBAP.00.DS.18.180).
dc.identifier.doi10.1002/mabi.201900109
dc.identifier.issn1616-5187
dc.identifier.issue8en_US
dc.identifier.pmid31222894
dc.identifier.scopus2-s2.0-85067867205
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1002/mabi.201900109
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5088
dc.identifier.volume19
dc.identifier.wosWOS:000479103500001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Muhammet
dc.institutionauthorAydın, Elif Burcu
dc.language.isoen
dc.publisherWiley-VCH Verlag
dc.relation.ispartofMacromolecular Bioscience
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectdisposable electrodes
dc.subjectelectrochemical impedance spectroscopy
dc.subjectgraft polymer
dc.subjectlung cancer
dc.subjectneuron-specific enolase
dc.subjectBiomarkers
dc.subjectCrosslinking
dc.subjectDiseases
dc.subjectElectrochemical impedance spectroscopy
dc.subjectGraft copolymers
dc.subjectGrafting (chemical)
dc.subjectImmunosensors
dc.subjectMonoclonal antibodies
dc.subjectNeurons
dc.subjectPlastic coatings
dc.subjectThiophene
dc.subjectTin oxides
dc.subjectDisposable electrodes
dc.subjectGlutaraldehyde cross-linking
dc.subjectImpedimetric immunosensors
dc.subjectIndium tin oxide electrodes
dc.subjectLinear detection ranges
dc.subjectLung Cancer
dc.subjectNeuron specific enolase
dc.subjectRelative standard deviations
dc.subjectElectrochemical electrodes
dc.subjectcopolymer
dc.subjectindium tin oxide
dc.subjectmonoclonal antibody
dc.subjectneuron specific enolase
dc.subjectpoly(thiophene) graft poly(methacrylamide) polymer
dc.subjecttumor marker
dc.subjectunclassified drug
dc.subjectacrylamide derivative
dc.subjectcross linking reagent
dc.subjectenolase
dc.subjectglutaraldehyde
dc.subjectmethacrylamide
dc.subjectmonoclonal antibody
dc.subjectpolymer
dc.subjectpolythiophene
dc.subjectthiophene derivative
dc.subjecttumor marker
dc.subjectArticle
dc.subjectatomic force microscopy
dc.subjectcyclic potentiometry
dc.subjectenergy dispersive X ray spectroscopy
dc.subjectenzyme immobilization
dc.subjectFourier transform infrared spectroscopy
dc.subjecthuman
dc.subjectimpedance spectroscopy
dc.subjectlimit of detection
dc.subjectmeasurement
dc.subjectprotein cross linking
dc.subjectproton nuclear magnetic resonance
dc.subjectRaman spectrometry
dc.subjectreproducibility
dc.subjectscanning electron microscopy
dc.subjectserum
dc.subjectsynthesis
dc.subjectblood
dc.subjectchemistry
dc.subjectelectrochemical analysis
dc.subjectelectrode
dc.subjectgenetic procedures
dc.subjectimmunoassay
dc.subjectpolymerization
dc.subjectprocedures
dc.subjectAcrylamides
dc.subjectAntibodies, Monoclonal
dc.subjectBiomarkers, Tumor
dc.subjectBiosensing Techniques
dc.subjectCross-Linking Reagents
dc.subjectElectrochemical Techniques
dc.subjectElectrodes
dc.subjectGlutaral
dc.subjectHumans
dc.subjectImmunoassay
dc.subjectLimit of Detection
dc.subjectPhosphopyruvate Hydratase
dc.subjectPolymerization
dc.subjectPolymers
dc.subjectReproducibility of Results
dc.subjectThiophenes
dc.titleA Highly Selective Poly(thiophene)-graft-Poly(methacrylamide) Polymer Modified ITO Electrode for Neuron Specific Enolase Detection in Human Serum
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
5088.pdf
Boyut:
3.43 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu