A Highly Selective Poly(thiophene)-graft-Poly(methacrylamide) Polymer Modified ITO Electrode for Neuron Specific Enolase Detection in Human Serum
Yükleniyor...
Dosyalar
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-VCH Verlag
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, an impedimetric immunosensor based on polymer poly(thiophene)-graft-poly(methacrylamide) polymer (P(Thi-g-MAm)) modified indium tin oxide (ITO) electrode is developed for the detection of the Neuron Specific Enolase (NSE) cancer biomarker. First, the P(Thi-g-MAm) polymer is synthesized and coated on the ITO electrode by using a spin-coating technique. P(Thi-g-MAm) polymer acts as an immobilization platform for immobilization of NSE-specific monoclonal antibodies. Anti-NSE antibodies are utilized as biosensing molecules and they bind to the amino groups of P(Thi-g-Mam) polymer via glutaraldehyde cross-linking. Spin-coating technique is employed for bioelectrode fabrication and this technique provides a thin and uniform film on the ITO electrode surface. This bioelectrode fabrication technique is simple and it generates a suitable platform for large-scale loadings of anti-NSE antibodies. This immunosensor exhibits a wide linear detection range from 0.02 to 4 pg mL?1 and with an ultralow detection limit of 6.1 fg mL?1. It reveals a good long-term stability (after 8 weeks, 78% of its initial activity), an excellent reproducibility (1.29% of relative standard deviation (RSD)), a good repeatability (5.55% of RSD), and a high selectivity. In addition, the developed immunosensor is proposed as a robust diagnostic tool for the clinical detection of NSE and other cancer biomarkers. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Açıklama
Anahtar Kelimeler
disposable electrodes, electrochemical impedance spectroscopy, graft polymer, lung cancer, neuron-specific enolase, Biomarkers, Crosslinking, Diseases, Electrochemical impedance spectroscopy, Graft copolymers, Grafting (chemical), Immunosensors, Monoclonal antibodies, Neurons, Plastic coatings, Thiophene, Tin oxides, Disposable electrodes, Glutaraldehyde cross-linking, Impedimetric immunosensors, Indium tin oxide electrodes, Linear detection ranges, Lung Cancer, Neuron specific enolase, Relative standard deviations, Electrochemical electrodes, copolymer, indium tin oxide, monoclonal antibody, neuron specific enolase, poly(thiophene) graft poly(methacrylamide) polymer, tumor marker, unclassified drug, acrylamide derivative, cross linking reagent, enolase, glutaraldehyde, methacrylamide, monoclonal antibody, polymer, polythiophene, thiophene derivative, tumor marker, Article, atomic force microscopy, cyclic potentiometry, energy dispersive X ray spectroscopy, enzyme immobilization, Fourier transform infrared spectroscopy, human, impedance spectroscopy, limit of detection, measurement, protein cross linking, proton nuclear magnetic resonance, Raman spectrometry, reproducibility, scanning electron microscopy, serum, synthesis, blood, chemistry, electrochemical analysis, electrode, genetic procedures, immunoassay, polymerization, procedures, Acrylamides, Antibodies, Monoclonal, Biomarkers, Tumor, Biosensing Techniques, Cross-Linking Reagents, Electrochemical Techniques, Electrodes, Glutaral, Humans, Immunoassay, Limit of Detection, Phosphopyruvate Hydratase, Polymerization, Polymers, Reproducibility of Results, Thiophenes
Kaynak
Macromolecular Bioscience
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
19
Sayı
8
