A Comparison of Ice Cold Water Pretreatment and alpha-Bromonaphthalene Cytogenetic Method for Identification of Papaver Species
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Dosyalar
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Hindawi Publishing Corporation
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The plants belonging to many species in genus Papaver are very similar and have very small chromosomes that make identification very difficult. The study aimed to compare the effects of alpha-bromonaphtalene and ice cold water pretreatment to identify chromosomes of Papaver species collected from different areas of Iranian West Azerbaijan and Turkish Van, Agri and, Hakkari provinces. The seeds were germinated in Jacobson trays at 24 degrees C under continuous light. Thereafter, roots from 1.5 cm long plantlets were pretreated with alpha bromonaphtalene for 15, 30, and 45min or at 0 degrees C in ice cold water for 24 h before fixing, hydrolyzation, and feulgen staining. The ice cold water pretreatment was more appropriate and easy to determine chromosomes. Seeds from seven samples did not germinate. Sixty samples out of the rest of 62 samples were identified as P. pseudo orientale, one sample was identified as P. bracteatum, and another as P. orientale. This is the first study that used ice cold water to determine the chromosomes in papaver species. It is hoped that it will also facilitate to determine chromosome number and identify other papver species.
Açıklama
Anahtar Kelimeler
Section Oxytona, Alkaloids
Kaynak
Scientific World Journal
WoS Q Değeri
Q2
Scopus Q Değeri
Q2